In this study, the gut microbiome of healthy adult honeybees, Apis cerana, was investigated by sequencing the V3 – V4 region in 16S rRNA gene using Illumina Miseq platform. The total of 37,853 reads for 16S rRNA gene were obtained and 30,121 (79.6%) reads were valid with 25,291 (84.0%) reads that were classified into 116 species belonging to four major phyla. The relative abundances of the bacterial isolates in honeybee samples were phylum Proteobacteria (70.7%), Actinobacteria (10.7%), Firmicutes (10.3%), and Bacteroidetes (8.4%), respectively. Lactic acid bacteria comprised 18.95% with 10 groups including Bifidobacterium asteroides, B. indicum, Fructobacillus fructosus, Lactobacillus apinorum, L. apis, L. helsingborgensis, L. kimbladii, L. kullabergensis, and L. kunkeei. The presence of beneficial bacteria in the gut highlighted their role in the honeybee and suggested that they can be promising candidates for the development of probiotics for health improvement, infection control and disease management of honeybees.

中文翻译：

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越南中华蜜蜂肠道菌群研究

在这项研究中，通过使用 Illumina Miseq 平台对 16S rRNA 基因中的 V3 - V4 区域进行测序，研究了健康成年蜜蜂（Apis cerana）的肠道微生物组。获得了 16S rRNA 基因的 37,853 个读数，其中 30,121 (79.6%) 个读数有效，其中 25,291 (84.0%) 个读数被分为属于四个主要门的 116 个物种。蜜蜂样品中细菌分离株的相对丰度分别为变形菌门 (70.7%)、放线菌门 (10.7%)、厚壁菌门 (10.3%) 和拟杆菌门 (8.4%)。乳酸菌占18.95%，包括星状双歧杆菌、B. indicum、果酸乳杆菌、松果乳杆菌、L. apis、L. helsingborgensis、L. kimbladii、L. kullabergensis和L. kunkeei等10个组。