Summary Emerging experimental framework suggests that plants under biotic stress may actively seek help from soil microbes, but empirical evidence underlying such a cry for help strategy is limited. We used integrated microbial community profiling, pathogen and plant transcriptive gene quantification and culture-based methods to systematically investigate a three-way interaction between the wheat plant, wheat-associated microbiomes and Fusarium pseudograminearum (Fp). A clear enrichment of a dominant bacterium, Stenotrophomonas rhizophila (SR80), was observed in both the rhizosphere and root endosphere of Fp-infected wheat. SR80 reached 3.7 . 107 cells g-1 in the rhizosphere and accounted for up to 11.4% of the microbes in the root endosphere. Its abundance had a positive linear correlation with the pathogen load at base stems and expression of multiple defense genes in top leaves. Upon re-introduction in soils, SR80 enhanced plant growth, both the below- and above-ground, and induced strong disease resistance by priming plant defense in the aboveground plant parts, but only when the pathogen was present Together, the bacterium SR80 seems to have acted as an early warning system for plant defense. This work provides novel evidence for the potential protection of plants against pathogens by an enriched beneficial microbe via modulation of the plant immune system.

中文翻译：

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植物募集有益微生物以抑制土壤传播病原体的证据

总结新兴的实验框架表明，处于生物胁迫下的植物可能会主动从土壤微生物中寻求帮助，但是这种寻求帮助的策略所依据的经验证据有限。我们使用整合的微生物群落特征分析，病原体和植物转录基因定量以及基于培养的方法，系统地研究了小麦植物，小麦相关微生物群和伪镰刀菌（Fp）之间的三向相互作用。在感染了Fp的小麦的根际和根内层均观察到明显富集优势细菌，嗜麦芽单胞菌（SR80）。SR80达到3.7。根际中有107个细胞g-1，占根内层微生物的11.4％。它的丰度与基茎上的病原体负荷和顶叶中多个防御基因的表达呈线性正相关。重新引入土壤后，SR80增强了地下和地下的植物生长，并通过在地上植物部分引发植物防御而诱导了强大的抗病性，但只有当病原体同时存在时，SR80细菌才似乎能够充当了植物防御的预警系统。这项工作提供了新的证据，证明了通过调节植物免疫系统，丰富的有益微生物可以潜在地保护植物免受病原体侵害。但是只有当病原体同时存在时，SR80细菌才似乎充当了植物防御的预警系统。这项工作提供了新的证据，表明通过调节植物免疫系统，丰富的有益微生物可以潜在地保护植物免受病原体侵害。但是只有当病原体同时存在时，SR80细菌才似乎充当了植物防御的预警系统。这项工作提供了新的证据，表明通过调节植物免疫系统，丰富的有益微生物可以潜在地保护植物免受病原体侵害。