Abstract

Controlling wound pain, absorption of wound exudate, and minimizing the frequency of changing the dressing are necessary parameters to facilitate wound-healing process. Hence, stable hydrophilic hydrogels applied in the form of film with the capability of wound exudate absorption and anti-inflammatory drug release may be used as an ideal wound dressing. Furthermore, physically crosslinked hydrogels are preferred over chemical ones because of being free from remaining harmful chemicals and adverse effects on the entrapped drugs. Thus, in this study, we developed an antibacterial bilayer membrane composed of a combination of polyvinyl alcohol/polyvinyl pyrrolidone (PVA/PVP) with the ability to release Ibuprofen (IBP). First, 1% IBP was incorporated in different ratios of PVA/PVP solution (90:10, 80:20, and 70:30); then, the hydrogels were crosslinked through repetitive freeze-thawing cycles followed by lyophilization, as the bottom layer. Second, the top layer was made in the same way but without the drug. Morphology, mechanical properties, swelling ratio, degradation rate, water vapor transmission rate (WVTR), and in vitro drug release of bilayer membranes were evaluated, then the content of PVP was optimized. The results indicated that the sample with the PVA/PVP ratio of 80:20 encompassed large and interconnected pores and also a proper swelling degree (500% in 48 h), weight loss (60% during 12 days) and WVTR (2721.1 ± 93.3 g/m²); thus, it is considered as the optimized membrane. MTT and antibacterial assays also showed acceptable viability and bactericidal activity for the membranes containing IBP. In conclusion, the prepared bilayer membrane can absorb wound exudate within the dressing matrix, release IBP locally, and keep its stability at the wound site. The bilayer membrane also exhibited an excellent antibacterial feature for accelerating the wound-healing process.

摘要

控制伤口疼痛、吸收伤口渗出液和尽量减少更换敷料的频率是促进伤口愈合过程的必要参数。因此，以薄膜形式应用的具有伤口渗出液吸收和抗炎药物释放能力的稳定亲水水凝胶可用作理想的伤口敷料。此外，物理交联的水凝胶比化学水凝胶更受欢迎，因为它没有残留的有害化学物质和对包裹的药物的不利影响。因此，在这项研究中，我们开发了一种抗菌双层膜，由聚乙烯醇/聚乙烯吡咯烷酮 (PVA/PVP) 的组合组成，具有释放布洛芬 (IBP) 的能力。首先，在不同比例的 PVA/PVP 溶液（90:10、80:20 和 70:30）中加入 1% IBP；然后，水凝胶通过反复冻融循环交联，然后冻干，作为底层。其次，顶层以相同的方式制作，但不含药物。形态、机械性能、溶胀率、降解率、水蒸气透过率 (WVTR) 和评价了双层膜的体外药物释放，进而优化了PVP的含量。结果表明，PVA/PVP 比为 80:20 的样品包含大且相互连接的孔隙，并且具有适当的溶胀度（48 小时内 500%）、失重（12 天内 60%）和 WVTR（2721.1 ± 93.3）克/米² ); 因此，它被认为是优化的膜。MTT 和抗菌试验也显示出含有 IBP 的膜具有可接受的活力和杀菌活性。总之，所制备的双层膜可以吸收敷料基质内的伤口渗出液，局部释放 IBP，并保持其在伤口部位的稳定性。双层膜还表现出优异的抗菌特性，可加速伤口愈合过程。