Recently, Lactococcus lactis subsp. lactis has been reported to have immunostimulating properties in an immunosuppressed-animal model. However, the immunological activities of Lactococcus lactis and the molecular mechanisms remain unclear. In this report, we evaluated the immunostimulating activity and associated mechanisms of Lactococcus lactis subsp. lactis GCWB1176 (GCWB1176) in macrophages and cyclophosphamide (CTX)-induced immunosuppressed mice. In a series of safety tests, GCWB1176 was found to have a negative response to hemolysis, as well as susceptibility to antibiotics. Administration of GCWB1176 elevated natural killer (NK) cell activities; concanavalin A-induced T cell proliferation; and serum levels of tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-2, IL-4, IL-10 and IL-12 in CTX-induced immunosuppressed mice. In RAW264.7 macrophages, treatment with GCWB1176 induced phagocytic activity and increased the production of nitric oxide (NO) and expression of inducible NO synthase. Simultaneously, GCWB1176 increased the production of TNF-α, IFN-γ, IL-1β, IL-10 and IL-12 from mouse splenocytes and RAW264.7 cells. In addition, GCWB1176 significantly increased the transcriptional activities of NF-κB and iNOS. Taken together, GCWB1176 improved immune function through the activation of macrophages and NK cells. These findings suggest that dietary supplementation of GCWB1176 may be used to enhance immunity.

中文翻译：

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乳酸乳球菌GCWB1176在环磷酰胺诱导的免疫抑制模型中的免疫调节活性。

最近，乳酸乳球菌亚种。乳酸已报告有免疫刺激在免疫抑制动物模型属性。然而，乳酸乳球菌的免疫活性和分子机制仍不清楚。在本报告中，我们评估了乳酸乳球菌亚种的免疫刺激活性及其相关机制。乳酸菌巨噬细胞和环磷酰胺（CTX）诱导的免疫抑制小鼠中的GCWB1176（GCWB1176）。在一系列安全测试中，GCWB1176被发现对溶血反应为阴性，并对抗生素敏感。施用GCWB1176可提高自然杀伤（NK）细胞的活性；伴刀豆球蛋白A诱导的T细胞增殖；CTX诱导的免疫抑制小鼠的肿瘤坏死因子（TNF）-α，干扰素（IFN）-γ，白介素（IL）-2，IL-4，IL-10和IL-12的水平 在RAW264.7巨噬细胞中，GCWB1176处理可诱导吞噬活性，并增加一氧化氮（NO）的产生和诱导型NO合酶的表达。同时，GCWB1176增加了小鼠脾细胞和RAW264.7细胞的TNF-α，IFN-γ，IL-1β，IL-10和IL-12的产生。此外，GCWB1176显着增加了NF-κB和iNOS的转录活性。综上所述，GCWB1176通过激活巨噬细胞和NK细胞改善了免疫功能。这些发现表明，饮食中补充GCWB1176可用于增强免疫力。