The most effective strategy to manage bean anthracnose (ANT), caused by Colletotrichum lindemuthianum, is the use of resistant cultivars. There are more than 20 ANT resistance genes that have been identified and mapped in common bean (Phaseolus vulgaris L.) chromosomes. Moreover, quantitative resistance loci (QRLs) have been described through genome‐wide association studies (GWAS). Identification of pathogen‐responsive genes and proteins on a molecular level provides a better understanding of metabolic pathways involved in ANT resistance. In this study, we investigated typical resistance proteins located close to ANT resistance loci in the common bean reference genome. Among them, we checked for proteins with nucleotide‐binding and leucine‐rich repeat (NBS‐LRR) domains and kinase domains since most resistance genes encode proteins with these domains. In addition, proteins with kinase domains are known to operate as pattern‐recognition receptors that recognize pathogen‐associated molecular patterns (PAMPs) and activate an immune response. Based on the common bean reference genome (Version 2.1), the regions 500‐kb upstream and downstream of the physical position of each ANT resistance locus were considered for a candidate gene search. Thus, an integrated map of ANT resistance loci and candidate genes (encoding defense response‐related proteins) was constructed. This map contains candidate genes for all ANT resistance genes and QRLs previously described in the literature. A total of 256 NBS‐LRR proteins and 200 protein kinases were detected. The functions of the identified candidate genes in ANT response should be validated and studies should be performed to understand how they interact with metabolic pathways.

中文翻译：

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菜豆基因组中炭疽病抗性基因座和RLK和NBS‐LRR编码基因的整合

处理由炭疽菌引起的豆炭疽病（ANT）的最有效策略是使用抗性品种。在普通豆（菜豆）中已鉴定并定位了20多个ANT抗性基因。L.）染色体。此外，已经通过全基因组关联研究（GWAS）描述了定量抗性基因座（QRL）。在分子水平上鉴定病原体反应性基因和蛋白质可更好地了解与ANT抗性有关的代谢途径。在这项研究中，我们调查了普通豆参考基因组中靠近ANT抗性基因座的典型抗性蛋白。其中，我们检查了具有核苷酸结合和富亮氨酸重复序列（NBS-LRR）结构域和激酶结构域的蛋白质，因为大多数抗性基因都编码具有这些结构域的蛋白质。此外，已知具有激酶结构域的蛋白质可作为模​​式识别受体，识别病原体相关的分子模式（PAMP）并激活免疫反应。基于普通bean参考基因组（2.1版），在每个ANT抗性基因座物理位置的上游和下游500 kb的区域被视为候选基因搜索。因此，构建了ANT抗性基因座和候选基因（编码防御反应相关蛋白）的整合图谱。该图包含先前文献中所述的所有ANT抗性基因和QRL的候选基因。总共检测到256个NBS-LRR蛋白和200个蛋白激酶。应该验证已确定的候选基因在ANT反应中的功能，并应进行研究以了解它们如何与代谢途径相互作用。绘制了ANT抗性基因座和候选基因（编码防御反应相关蛋白）的整合图谱。该图包含先前文献中所述的所有ANT抗性基因和QRL的候选基因。总共检测到256个NBS-LRR蛋白和200个蛋白激酶。应当验证已鉴定的候选基因在ANT反应中的功能，并应进行研究以了解它们如何与代谢途径相互作用。绘制了ANT抗性基因座和候选基因（编码防御反应相关蛋白）的整合图谱。该图包含先前文献中所述的所有ANT抗性基因和QRL的候选基因。总共检测到256个NBS-LRR蛋白和200个蛋白激酶。应该验证已确定的候选基因在ANT反应中的功能，并应进行研究以了解它们如何与代谢途径相互作用。