Rabbit meat is considered as an economic source of white meat, increasing its production is limited by the buck fertility, since one rabbit can be used to inseminate up to 15 female. The aim of the current study is to enhance male rabbit fertility by using dietary antioxidants including Aloe vera gel (AVG) and pomegranate peels (PP). In a 60 days experiment, 48 V‐Line 5‐month‐old rabbit bucks of average body weight (2,300 ± 20) kg were allocated into four dietary treatments (n = 12/group) as follow: CON (fed on control diet), ALOE (received AVG in drinking water; 500 mg/L drinking water), POM3 (fed on basal diet + 3% of pomegranate) and POM5 (fed on basal diet + 5% of pomegranate). Semen samples were collected at d30 and d60 of the experiment and used for analysis of semen quality. Sexual behaviour was reported in terms of latency to first mating and ejaculation interval. At the end of the experiment, six bucks were euthanized from each group, blood samples were collected and used for testosterone level determination and testicular tissue samples were collected and used for key antioxidant and spermatogenesis enzymes assessment, and testes histopathological evaluation. The UNIVARIATE procedures of SAS 9.4 were used to analyse the data, significance was declared at p ≤.05. PP supplementation improved percentage of progressive motile sperms while AVG negatively impacted it (p = .04), sperm concentration and metabolically active sperm cells were the highest in PP and lowest in ALOE supplemented bucks (p = .01 and .01; respectively). Testicular alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) decreased in AVG supplemented group (p = .01 and .02; respectively). From our findings, AVG in its fresh form decreased fertility of rabbit bucks, while PP is potent fertility boosting for rabbit bucks.

中文翻译：

---

膳食石榴皮 (Punica granatum L.) 和芦荟凝胶 (Aloe barbadensis miller) 补充剂对成熟 V-Line 兔雄鹿睾丸抗氧化生物标志物和精子发生酶的影响。

兔肉被认为是白肉的经济来源，增加其产量受到雄鹿生育力的限制，因为一只兔子可以用来给多达 15 只雌性兔子授精。当前研究的目的是通过使用包括芦荟凝胶 ( AVG ) 和石榴皮 ( PP )在内的膳食抗氧化剂来提高雄性兔的生育能力。在 60 天的实验中，将 48 只平均体重 (2,300 ± 20) kg 的 V-Line 5 个月大兔子分配到以下四种饮食处理中（n  = 12/组）：CON（以对照饮食喂养） ,芦荟（在饮用水中获得 AVG；500 毫克/升饮用水）、POM3（以基础饮食喂养 + 3% 的石榴）和POM5（以基础饮食 + 5% 的石榴喂养）。在实验的第 30 天和第 60 天收集精液样本，用于分析精液质量。性行为是根据第一次交配和射精间隔的潜伏期来报告的。实验结束时对每组6块钱进行安乐死，采集血样用于睾酮水平测定，采集睾丸组织样品用于关键抗氧化和生精酶评估，以及睾丸组织病理学评估。SAS 9.4 的 UNIVARIATE 程序用于分析数据，在p  ≤ .05处声明显着性。PP 补充剂提高了进行性活动精子的百分比，而 AVG 对其产生了负面影响（p = .04)，精子浓度和代谢活跃的精子细胞在 PP 中最高，在补充芦荟的雄鹿中最低（ 分别为p = .01 和 .01；）。AVG 补充组的睾丸碱性磷酸酶 (ALP) 和乳酸脱氢酶 (LDH) 降低（ 分别为p = .01 和 .02；）。根据我们的研究结果，新鲜形式的 AVG 会降低兔雄鹿的生育能力，而 PP 则能有效提高兔雄鹿的生育能力。