Gyrodactylids are ubiquitous ectoparasites of teleost fish, but our understanding of the host immune response against them is fragmentary. Here, we used RNA‐Seq to investigate genes involved in the primary response to infection with Gyrodactylus bullatarudis on the skin of guppies, Poecilia reticulata, an important evolutionary model, but also one of the most common fish in the global ornamental trade. Analysis of differentially expressed genes identified several immune‐related categories, including IL‐17 signalling pathway and Th17 cell differentiation, cytokine‐cytokine receptor interaction, chemokine signalling pathway, NOD‐like receptor signalling pathway, natural killer cell‐mediated cytotoxicity and pathways involved in antigen recognition, processing and presentation. Components of both the innate and the adaptive immune responses play a role in response to gyrodactylid infection. Genes involved in IL‐17/Th17 response were particularly enriched among differentially expressed genes, suggesting a significant role for this pathway in fish responses to ectoparasites. Our results revealed a sizable list of genes potentially involved in the teleost‐gyrodactylid immune response.

中文翻译：

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RNA序列分析的孔雀鱼对大叶小球菌感染的孔雀鱼免疫反应。

绞线虫是硬骨鱼普遍存在的外寄生物，但是我们对宿主针对它们的免疫反应的理解是零碎的。在这里，我们使用RNA‐Seq来研究与孔雀鱼（Poecilia reticulata）皮肤上的孔雀假单胞菌感染有关的主要反应基因。是重要的进化模型，也是全球观赏贸易中最常见的鱼类之一。差异表达基因的分析确定了几种与免疫相关的类别，包括IL-17信号通路和Th17细胞分化，细胞因子-细胞因子受体相互作用，趋化因子信号通路，NOD样受体信号通路，自然杀伤细胞介导的细胞毒性和涉及的通路抗原识别，加工和呈递。先天性和适应性免疫反应的组成部分都在对陀螺的感染中起一定作用。IL-17 / Th17应答中涉及的基因在差异表达基因中特别丰富，表明该途径在鱼类对体外寄生虫的应答中起着重要作用。