Myxozoans usually have a complex life cycle involving indirect transmission between vertebrate and invertebrate hosts. The vertical transmission of these parasites in vertebrate hosts has not been documented so far. Here, we assessed whether the myxozoan parasite Myxobolus honghuensis is vertically transmitted in naturally infected allogynogenetic gibel carp Carassius auratus gibelio (Bloch). M. honghuensis infection of broodfish, fertilized eggs and laboratory‐cultured progeny was monitored in 2018 and 2019. The presporogonic stage was microscopically observed in the pharynx of broodfish and their progeny. In situ hybridization confirmed the presence of M. honghuensis presporogonic stage in the pharynx of broodfish and progeny. Nested PCR results showed that M. honghuensis was present in tissues and eggs of broodfish, fertilized eggs and their corresponding progeny. The sequences obtained from broodfish and progeny showed 98.0−99.8% similarity with ITS‐5.8S rDNA of M. honghuensis. This study provides molecular and light microscopy evidence for the transfer of M. honghuensis from broodfish to progeny via the eggs, but it is insufficient to assert that M. honghuensis can transmit vertically in naturally infected allogynogenetic gibel carp. This is the first record about vertical transfer of myxozoan in the vertebrate host.

中文翻译：

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分子和光学显微镜证据证明洪湖蜜蜡菌从Car鱼亲鱼转移到后代。

粘虫动物通常具有复杂的生命周期，涉及脊椎动物和无脊椎动物宿主之间的间接传播。这些寄生虫在脊椎动物宿主中的垂直传播迄今尚未见文献记载。在这里，我们评估了是否在自然感染的同种异体成年长鳍carp鱼Car鱼（Bloch）中垂直传播了粘虫寄生虫洪湖My。在2018年和2019年监测了洪湖支原体对亲鱼，受精卵和实验室培养后代的感染。在亲鱼及其后代的咽部显微镜下观察到了繁殖前阶段。原位杂交证实洪湖山毛。亲鱼和后代的咽部处于生殖器前期。巢式PCR结果表明，洪湖分枝杆菌存在于亲鱼，受精卵及其后代的组织和卵中。从亲鱼和后代获得的序列表明98.0-99.8％的相似性与ITS-5.8S rDNA的M. honghuensis。这项研究为分子生物学和光学显微镜研究提供了证据，证明洪湖中华绒螯蟹是通过卵从亲鱼转移到后代的，但不足以断言洪湖中华绒螯蟹可以在自然感染的同种异体长鳍金枪鱼中垂直传播。这是关于粘虫在脊椎动物宿主中垂直转移的第一个记录。