This work aims to characterize the mixing and suspension dynamics occurring within two commercially available DASGIP bioreactor configurations, equipped with a two‐blade paddle impeller with large impeller to tank diameter ratio, D/T = 0.97. Both continuous and intermittent agitation modes were employed to determine the impact that agitation strategy has upon mass transfer and microcarrier settling/suspension. This paper builds upon the flow dynamics data presented in Part 1 for a flat bottom DASGIP bioreactor and shows how intermittent agitation can break‐up regions of slow mixing observed during continuous agitation, therefore substantially increasing the mixing efficiency of the system. Similarly, it was found that microcarrier characteristics might significantly affect the level of suspension when the impeller is in dwell status when intermittent agitation modes are used.

中文翻译：

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DASGIP生物反应器中的流动，悬浮和混合动力学，第2部分

这项工作的目的是表征在两个市售DASGIP生物反应器配置中发生的混合和悬浮动力学，该配置配备了两叶桨叶轮，叶轮与水箱的直径比大，D / T =0.97。连续和间歇搅拌模式均用于确定搅拌策略对传质和微载体沉降/悬浮的影响。本文基于第1部分中介绍的平底DASGIP生物反应器的流动动力学数据，显示了间歇性搅拌如何破坏连续搅拌过程中观察到的缓慢混合区域，从而显着提高了系统的混合效率。类似地，发现当使用间歇搅拌模式时，当叶轮处于停留状态时，微载体特性可能会显着影响悬浮液的水平。