It was extremely urgent to develop some simultaneous and sensitive biosensors for detecting multiplex serum tumor markers (TMs) for early screening of cancers. Herein, a multiplex assay was developed based on the DNA-LaMnO₃ (DNA-LMO) perovskite encoded probes and targets mediated competitive replacement strategy. Alpha fetoprotein (AFP), carcinoembryonic antigen (CEA) and prostate specific antigen (PSA) markers were employed as representative target TMs. Aptasensor is prepared by a series of DNA-LMO-M encode probes which were prepared by three hyperbranched DNA firstly immobilized on LMO encapsulating Pb, Cd or Cu ions. Then, three TMs aptamers were labeled on the stirring-rod and hybridized with the probes. After the developed encoded probes was incubated the TMs, the encoded probes corresponding to different TMs can be released into the supernatant through the competitive replacement. The inner metal ion can be simultaneously detected by square wave voltammetry corresponding to various TMs. Since the stirring rod can enrich many encoded probes containing a lot of metal ions, multiplex signal amplification can be realized. Due to the enrichment and easy separation of the stirring rod, the signal-to-noise ratio was also obviously improved and thus to results in good sensitivity and accuracy. Moreover, it took only 20 min to detect three targets which much faster than many same types of aptasensor. Under the optimal conditions, the low detection limit for CEA (3.6 × 10^-4 ng/mL), AFP (3.4 × 10^-4 ng/mL) and PSA (2.8 × 10^-4 ng/mL) were obtained. Therefore, this method is likely to be used for early and sensitive screening of tumors.

迫切需要开发一些同时灵敏的生物传感器来检测多重血清肿瘤标志物（TMs），以进行早期癌症筛查。在此，基于DNA-LaMnO _3进行了多重分析（DNA-LMO）钙钛矿编码的探针和靶标介导的竞争性替代策略。甲胎蛋白（AFP），癌胚抗原（CEA）和前列腺特异抗原（PSA）标记物用作代表性靶标TM。Aptasensor由一系列DNA-LMO-M编码探针制备，该探针由首先固定在封装Pb，Cd或Cu离子的LMO上的三个超支化DNA制备。然后，将三个TM适体标记在搅拌棒上并与探针杂交。将显影的编码探针孵育TM后，可以通过竞争性替换将对应于不同TM的编码探针释放到上清液中。内金属离子可以通过对应于各种TM的方波伏安法同时检测。由于搅拌棒可以富集许多包含许多金属离子的编码探针，因此可以实现多路信号放大。由于搅拌棒的富集和易于分离，信噪比也得到了明显改善，因此具有良好的灵敏度和准确性。而且，只花了20分钟即可检测到三个目标，这比许多相同类型的适体传感器要快得多。在最佳条件下，CEA的低检测限（3.6×10^获得了-4  ng / mL），AFP（3.4×10 ^-4  ng / mL）和PSA（2.8×10 ^-4  ng / mL）。因此，该方法可能用于早期和敏感的肿瘤筛查。