The Household Air Pollution Intervention Network (HAPIN) trial is evaluating health benefits of a liquefied petroleum gas (LPG) stove intervention in biomass cook-fuel using homes (n = 3200) in four low-and middle-income countries (LMICs) that include Peru, Guatemala, Rwanda and India. Longitudinal urine samples (n = 6000) collected from enrolled pregnant women, infants and older women will be analyzed for biomarkers associated with exposure and health outcomes. We report results from cross-validation of a lower cost high-performance liquid chromatography with fluorescence detection (HPLC-FLD) method with a higher resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the measurement of 1-hydroxypyrene (1PYR) and 2-naphthol (2NAP). Twenty-five split urine samples were analyzed by HPLC-FLD method at the India trial site in Chennai, India and by LC-MSMS method at the trial wide Biomarker Coordinating Center, Emory University, USA. The limits of detection (LOD) for the HPLC-FLD method were 0.02 ng/mL and 0.07 ng/mL for 2NAP and 1PYR, respectively. Bland-Altman analysis estimated a bias of 2.98 ng/ml for 2NAP (95% CI: −5.22, −0.75) and 0.09 ng/mL for 1PYR (95% CI: −0.02, 0.21) with HPLC-FLD levels being lower than LC-MSMS levels at higher concentrations. Analyses of additional urine samples (n = 119) collected during the formative phase of the HAPIN trial in India, showed 2NAP and 1PYR levels to be consistently above the limit of quantification (LOQ) and demonstrated the applicability of the method. The HPLC-FLD method can serve as a cost-effective and reliable analytical method to measure 2NAP and 1PYR in human urine in LMICs, within and beyond the HAPIN trial.

家庭空气污染干预网络 (HAPIN) 试验正在评估四个低收入和中等收入国家 (LMIC) 使用生物质烹饪燃料的家庭 (n = 3200) 液化石油气 (LPG) 炉灶干预的健康益处，其中包括秘鲁、危地马拉、卢旺达和印度。对从登记的孕妇、婴儿和老年妇女收集的纵向尿液样本 (n = 6000) 进行分析，以了解与暴露和健康结果相关的生物标志物。我们报告了用于测量 1-羟基芘的低成本高效液相色谱荧光检测 (HPLC-FLD) 方法与更高分辨率液相色谱-串联质谱 (LC-MS/MS) 方法的交叉验证结果(1PYR) 和 2-萘酚 (2NAP)。在印度钦奈的印度试验中心采用 HPLC-FLD 方法分析了 25 份尿液样本，并在美国埃默里大学生物标志物协调中心的试验范围内采用 LC-MSMS 方法进行了分析。 2NAP 和 1PYR 的 HPLC-FLD 方法的检测限 (LOD) 分别为 0.02 ng/mL 和 0.07 ng/mL。 Bland-Altman 分析估计 2NAP 的偏差为 2.98 ng/ml（95% CI：-5.22，-0.75），1PYR 的偏差为 0.09 ng/mL（95% CI：-0.02，0.21），且 HPLC-FLD 水平低于较高浓度的 LC-MSMS 水平。对印度 HAPIN 试验形成阶段收集的其他尿液样本 (n = 119) 的分析显示，2NAP 和 1PYR 水平始终高于定量限 (LOQ)，并证明了该方法的适用性。 HPLC-FLD 方法可以作为一种经济有效且可靠的分析方法，用于在 HAPIN 试验内外测量 LMIC 人尿液中的 2NAP 和 1PYR。