As invertebrates, shrimp are generally thought to solely rely on their innate immune system to combat invading pathogens. Recently, an increasing number of studies have revealed that the innate immune response of invertebrates exhibits diversity and specificity based on their diverse immune molecules. Herein, a full-length transcriptome analysis of several immune-related tissues (hepatopancreas, gill, hemocytes, stomach and intestine) in the kuruma shrimp (Marsupenaeus japonicus) was conducted to identify immune-related molecules with a focus on transcript variations. In total, 11,222 nonredundant full-length transcripts with an N50 length of 5174 were obtained, and most of these transcripts (94.84%) were successfully annotated. In addition, a total of 147 long noncoding RNAs (lncRNAs) were also predicted. Importantly, transcript variants of several vital immune-related genes were observed, including twenty-five alpha-2-macroglobulins (α2-Ms), ten Toll-like receptors (TLRs), six C-type lectins (CTLs), five M-type lectins (MTLs) and three Down syndrome cell adhesion molecules (Dscams). Furthermore, 509 nonredundant full-length transcripts were predicted to be generated from alternative splicing (AS) events, which contribute to the diversity of immune molecules. Overall, our study provides valuable data on the full-length transcripts of M. japonicus, which will facilitate the exploration of immune molecules in this species. Moreover, numerous transcript variants of immune molecules detected in this study provide clues for further investigating the diversity and specificity of the innate immune response in shrimp.

作为无脊椎动物，通常认为虾仅依靠其先天免疫系统来抵抗入侵的病原体。最近，越来越多的研究表明，无脊椎动物的先天免疫应答基于其多样的免疫分子而表现出多样性和特异性。本文对黑熊虾（Marsupenaeus japonicus）中几种免疫相关组织（肝胰脏，腮，血细胞，胃和肠）的全长转录组分析）进行了研究，以鉴定与免疫相关的分子，重点是转录本变异。总共获得了11222个N50长度的非冗余全长转录本，其中大多数转录本（94.84％）被成功注释。此外，还预测了总共147个长非编码RNA（lncRNA）。重要的是，观察到了几个重要的免疫相关基因的转录物变体，包括25个α-2-巨球蛋白（α2-Ms），十个Toll样受体（TLR），六个C型凝集素（CTL），五个M-类型的凝集素（MTL）和三个唐氏综合症细胞粘附分子（Dscams）。此外，预测从替代剪接（AS）事件产生509个非冗余全长转录本，这有助于免疫分子的多样性。总体，中号。japonicus，这将有助于探索该物种中的免疫分子。此外，在这项研究中检测到的许多免疫分子的转录变异体为进一步研究虾固有免疫应答的多样性和特异性提供了线索。