Electric fields (EFs) have shown promising impact on wound healing, these alone are ineffective to stimulate the whole wound area. In this study, we developed a newly regenerated bacterial cellulose/polypyrrole/carbon nanotube (rBC/PPy/CNT) electroactive hydrogel through cellulose dissolution, and physical and chemical crosslinking method to enhance cell proliferation with EF for wound healing. The hydrogels were characterized with FESEM, FTIR, XRD, TGA, conductivity, mechanical, and swelling tests. The results showed that PPy and CNTs were successfully deposited in the rBC/PPy/CNT hydrogels, which exhibited excellent thermal stability, mechanical strength, recoverability, swelling ability, and demonstra-ted 10⁷ fold higher electrical conductivity than rBC. In vitro analysis proved good biocompatibility of rBC/PPy/CNT whereon NIH3T3 cells proliferated evidently. Especially, the combination of EF with rBC/PPy/CNT significantly enhanced the cell proliferation as compared to rBC (p < 0.05). The overall results suggest the promising potential of rBC/PPy/CNT combined with EF for enhancing cellular activities in wound healing.

电场（EFs）对伤口愈合显示出可喜的影响，仅电场对刺激整个伤口区域无效。在这项研究中，我们开发了一种新的再生细菌细菌纤维素/聚吡咯/碳纳米管（rBC / PPy / CNT）电活性水凝胶，通过纤维素溶解以及物理和化学交联方法来增强EF促进伤口愈合的细胞增殖。通过FESEM，FTIR，XRD，TGA，电导率，机械和溶胀测试对水凝胶进行了表征。结果表明，PPy和CNTs成功地沉积在rBC / PPy / CNT水凝胶中，其表现出优异的热稳定性，机械强度，可恢复性，溶胀能力，并且电导率比rBC高10 ⁷倍。体外分析证明rBC / PPy / CNT具有良好的生物相容性，NIH3T3细胞在该细胞上明显增殖。尤其是，EF与rBC / PPy / CNT的组合与rBC相比显着增强了细胞增殖（p  <0.05）。总体结果表明，rBC / PPy / CNT与EF结合使用有望在伤口愈合中增强细胞活性。