A novel electrochemical nanobiosensor for the detection of miR-155 (as breast cancer biomarker) is introduced . Fe3O4NPs@Ag core-shell nanoparticles were synthesized and their shape and characteristics were confirmed by scanning electron microscope (SEM) imaging, Fourier-transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) methods. Synthesized nanoparticles were applied onto the magnetic bar carbon paste electrode and then the amine-modified anti-miR-155 (single-stranded probes) was applied on the modified electrode surface and upon hybridization with target miR-155, resveratrol (RSV) was eventually applied as an electrochemical label on the double-strand oligonucleotide. Differential pulse voltammetry (DPV) of the oxidation peak of RSV was assumed as the final signal by sweeping potential from 0 to 0.6 V (vs. Ag/AgCl). The fabrication process was optimized through a series of experiments and the optimized process was confirmed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The linear range of the fabricated nanobiosensor was 0.5 fM to 1.0 nM and the detection limit was 0.15 fM. The nanobiosensor was able to pass reproducibility and specificity tests using different types of mismatched target sequences.Spiked real samples of human serum were used to confirm that the nanobiosensor enables detection of miR-155 without any significant interferences from other moieties and molecules. Finally, the molecular dynamics simulation of the RSV interaction with single- and double-stranded oligonucleotide was performed and confirmed the preferential binding of RSV to double-stranded DNA; therefore, it can be used as the electrochemical label of DNA and/or miRNA hybridization–based biosensors. Graphical abstract Graphical abstract

中文翻译：

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使用磁性核壳纳米粒子通过白藜芦醇与核酸的相互作用检测 miR-155 的实验和理论研究

介绍了一种用于检测 miR-155（作为乳腺癌生物标志物）的新型电化学纳米生物传感器。合成了 Fe3O4NPs@Ag 核壳纳米粒子，并通过扫描电子显微镜 (SEM) 成像、傅里叶变换红外光谱 (FTIR) 和 X 射线衍射 (XRD) 方法确认了它们的形状和特性。将合成的纳米粒子施加到磁棒碳糊电极上，然后将胺修饰的抗 miR-155（单链探针）施加到修饰的电极表面，与目标 miR-155 杂交后，最终得到白藜芦醇（RSV）用作双链寡核苷酸上的电化学标记。RSV 氧化峰的微分脉冲伏安法 (DPV) 被假定为通过从 0 到 0.6 V（相对于 Ag/AgCl）扫描电位的最终信号。通过一系列实验优化了制造工艺，并使用循环伏安法 (CV) 和电化学阻抗谱 (EIS) 确认了优化工艺。制备的纳米生物传感器的线性范围为 0.5 fM 至 1.0 nM，检测限为 0.15 fM。纳米生物传感器能够通过使用不同类型错配目标序列的再现性和特异性测试。最后，进行了 RSV 与单链和双链寡核苷酸相互作用的分子动力学模拟，并证实了 RSV 与双链 DNA 的优先结合；所以，它可以用作基于 DNA 和/或 miRNA 杂交的生物传感器的电化学标记。图形摘要图形摘要