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You get what you screen for: on the value of fermentation characterization in high-throughput strain improvements in industrial settings.
Journal of Industrial Microbiology & Biotechnology ( IF 3.2 ) Pub Date : 2020-08-02 , DOI: 10.1007/s10295-020-02295-3
Maren Wehrs 1 , Alexander de Beaumont-Felt 1 , Alexi Goranov 1 , Patrick Harrigan 1 , Stefan de Kok 1 , Sarah Lieder 1 , Jim Vallandingham 1 , Kristina Tyner 1
Affiliation  

While design and high-throughput build approaches in biotechnology have increasingly gained attention over the past decade, approaches to test strain performance in high-throughput have received less discussion in the literature. Here, we describe how fermentation characterization can be used to improve the overall efficiency of high-throughput DBTAL (design-build-test-analyze-learn) cycles in an industrial context. Fermentation characterization comprises an in-depth study of strain performance in a bioreactor setting and involves semi-frequent sampling and analytical measurement of substrates, cell densities and viabilities, and (by)products. We describe how fermentation characterization can be used to (1) improve (high-throughput) strain design approaches; (2) enable the development of bench-scale fermentation processes compatible with a wide diversity of strains; and (3) inform the development of high-throughput plate-based strain testing procedures for improved performance at larger scales.



中文翻译:

您将获得筛查的内容:发酵表征在工业设置中提高高通量菌株的价值。

尽管在过去的十年中,生物技术中的设计和高通量构建方法越来越受到关注,但在高通量中测试菌株性能的方法在文献中却鲜有讨论。在这里,我们描述了如何在工业环境中使用发酵表征技术来提高高通量DBTAL(设计,构建,测试,分析,学习)循环的整体效率。发酵表征包括在生物反应器环境中对菌株性能的深入研究,涉及半频繁的底物,细胞密度和活力以及(副)产物的采样和分析测量。我们描述了如何将发酵表征用于(1)改进(高通量)菌株设计方法;(2)能够开发与多种菌株兼容的实验室规模发酵工艺;(3)指导开发基于高通量板的应变测试程序,以提高大规模生产的性能。

更新日期:2020-08-02
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