While design and high-throughput build approaches in biotechnology have increasingly gained attention over the past decade, approaches to test strain performance in high-throughput have received less discussion in the literature. Here, we describe how fermentation characterization can be used to improve the overall efficiency of high-throughput DBTAL (design-build-test-analyze-learn) cycles in an industrial context. Fermentation characterization comprises an in-depth study of strain performance in a bioreactor setting and involves semi-frequent sampling and analytical measurement of substrates, cell densities and viabilities, and (by)products. We describe how fermentation characterization can be used to (1) improve (high-throughput) strain design approaches; (2) enable the development of bench-scale fermentation processes compatible with a wide diversity of strains; and (3) inform the development of high-throughput plate-based strain testing procedures for improved performance at larger scales.

尽管在过去的十年中，生物技术中的设计和高通量构建方法越来越受到关注，但在高通量中测试菌株性能的方法在文献中却鲜有讨论。在这里，我们描述了如何在工业环境中使用发酵表征技术来提高高通量DBTAL（设计，构建，测试，分析，学习）循环的整体效率。发酵表征包括在生物反应器环境中对菌株性能的深入研究，涉及半频繁的底物，细胞密度和活力以及（副）产物的采样和分析测量。我们描述了如何将发酵表征用于（1）改进（高通量）菌株设计方法；（2）能够开发与多种菌株兼容的实验室规模发酵工艺；（3）指导开发基于高通量板的应变测试程序，以提高大规模生产的性能。