Cyanobacteria are able to synthesize high-value compounds, such as pigments, known for their bioactivities and several industrial uses. One of the key points for the extraction of pigments is solvent selection. Solvent interferes on pigments concentration, thus affecting the bioactive potential of the extracts. In this work, using frozen-dried biomass of Cyanobium sp. LEGE 06113, organic and aqueous pigment-rich extracts were obtained by a classic extraction methodology using four solvents — acetone (A), ethyl acetate (EA), ethanol (E) and water (W). In order to increase the efficiency of extraction from the cyanobacterium biomass, successive extractions were performed by using water after organic extraction (A-W, E-W, EA-W) and acetone after the aqueous extraction (W-A). Extraction yield and profile of carotenoids, phycobiliproteins, and phenolic compounds were quantified. The bioactive potential of Cyanobium sp. extracts was assessed in terms of antioxidant capacity (ABTS^•+, ^•NO, O₂^•− scavenging), anti-inflammatory capacity (COX inhibition), and cytotoxicity (HepG2). W-A showed the higher antioxidant capacity and higher content in carotenoids. E-W showed the highest content in phycobiliproteins and great antioxidant capacity. In terms of anti-inflammatory capacity, 100 μg_E mL⁻¹ of E-W extract exhibited capacity to inhibit both COX-1 and COX-2 enzymes. Finally, in what concerns the cytotoxic evaluation, E, W, A-W, E-W, and EA-W revealed to have no cytotoxic effects in concentrations up to 750 μg_E mL⁻¹. Overall, this work constitutes a valid contribution for the valorisation of Cyanobium sp. pigment-rich extracts for biotechnological applications.

蓝细菌能够合成以其生物活性和多种工业用途而闻名的高价值化合物，例如颜料。提取颜料的关键点之一是溶剂的选择。溶剂会干扰颜料的浓度，从而影响提取物的生物活性。在这项工作中，使用蓝藻的冷冻干燥生物质sp。LEGE 06113通过经典的提取方法，使用四种溶剂-丙酮（A），乙酸乙酯（EA），乙醇（E）和水（W）获得了富含有机和水性颜料的提取物。为了提高从蓝藻生物质中提取的效率，在有机萃取（AW，EW，EA-W）后用水和水萃取（WA）后的丙酮进行​​连续萃取。定量提取类胡萝卜素，藻胆蛋白和酚类化合物的提取量和概况。Cyanobium sp。的生物活性潜能。提取物根据抗氧化剂能力进行了评估（ABTS ^•+，^• NO，O ₂ ^•-清除），抗炎能力（抑制COX）和细胞毒性（HepG2）。WA显示出较高的抗氧化能力和较高的类胡萝卜素含量。EW显示藻胆蛋白含量最高，抗氧化能力强。在的抗炎能力方面，100微克_È毫升^-1 EW提取物显示出容量同时抑制COX-1和COX-2酶。最后，在所关注的细胞毒性评估，E，W，AW，EW，和EA-W透露，在浓度无细胞毒性作用高达750微克_è毫升^-1。总体而言，这项工作为Cyanobium sp。的增值提供了有效的贡献。用于生物技术应用的富含色素的提取物。