The use of fed-batch extractive fermentation can overcome inhibitory effects caused by the substrate and ethanol to the yeast cells, since it allows regulate the substrate concentration and remove the product as it is produced. The present study describes the modelling and experimental validation of ethanol production in fed-batch extractive fermentation with in situ ethanol removal by oleic acid in a non-conventional drop column bioreactor (DCB) operated under industrial conditions. The model developed using the hybrid Andrews–Levenspiel equation and ethanol distribution coefficient parameter (K_DE) provided an excellent description of the fed-batch extractive ethanol fermentation process with oleic acid. Furthermore, extractive fed-batch fermentation allowed the feed up to 306.6 kg m⁻³ of substrate (total reducing sugars), with total ethanol concentration in extractive fermentation in the ranging 100.3–139.8 kg m⁻³ (12.7–17.7 ºGL), 19.9–67.2% higher when compared with the conventional process without ethanol removal. Moreover, this process has the advantage of less effluent generated and energy consumption for ethanol recovery when compared to the conventional process.

补料分批提取发酵的使用可以克服底物和乙醇对酵母细胞造成的抑制作用，因为它可以调节底物浓度并在产生产物时去除产物。本研究描述了在工业条件下操作的非常规下降柱生物反应器 (DCB) 中，通过油酸原位去除乙醇的补料分批提取发酵中乙醇生产的建模和实验验证。使用混合 Andrews-Levenspiel 方程和乙醇分配系数参数 ( K _DE )开发的模型很好地描述了油酸的补料分批提取乙醇发酵过程。此外，提取补料分批发酵允许进料高达 306.6 kg m^-3的底物（总还原糖），提取发酵中的总乙醇浓度范围为 100.3–139.8 kg m ^-3 (12.7–17.7 ºGL)，与不去除乙醇的传统工艺相比，高 19.9–67.2%。此外，与传统工艺相比，该工艺具有废水产生量和乙醇回收能耗少的优势。