The objective of this study was to determine the influence of various concentrations of emerging pollutants (EPs) on growth, cell composition, and enzymatic hydrolysis of biomass. The microalgae used were Spirulina platensis LEB-52, Chlorella homosphaera, and Scenedesmus obliquus. The EPs used were paracetamol, diazepam, fluoxetine, acetylsalicylic acid, and caffeine, added to the cultures in concentrations ranging from 1 to 100 mg L⁻¹. The tests were carried out in closed 150-mL Erlenmeyer bioreactors, containing standard medium from each microalga culture. Cell concentrations were determined every 24 h using optical density. Culture continued for 20 days. At the end of the growth, biomass was collected and used to measure carbohydrates, proteins, and enzymatic hydrolysis, to determine possible changes due to the presence of EPs. In general, microalgae resisted concentrations of up to 100 mg L⁻¹ of paracetamol, acetylsalicylic acid, and caffeine, 30 mg L⁻¹ of diazepam, and 1 mg L⁻¹ of fluoxetine. S. obliquus was the most resistant strain, followed by C. homosphaera and S. platensis LEB-52 being less resistant. Acetylsalicylic acid and caffeine influenced the content of carbohydrates and proteins in biomass, reaching values ​above 35% of carbohydrates for S. platensis LEB-52 and above 66% of proteins for S. obliquus. There was no influence of EPs in the enzymatic hydrolysis processes. Microalgae showed resistance to EP concentrations, thereby emerging as a promising wastewater bioremediation technology. These high levels of carbohydrates are of great value because they can be used in the production of bioethanol.

这项研究的目的是确定各种浓度的新兴污染物（EPs）对生物量的生长，细胞组成和酶水解的影响。使用的微藻是螺旋藻LEB-52，小球藻和斜生藻。所使用的EP为扑热息痛，地西epa，氟西汀，乙酰水杨酸和咖啡因，其浓度范围为1至100 mg L ^-1。测试在封闭的150 mL锥形生物反应器中进行，该反应器包含每种微藻培养物中的标准培养基。每24小时使用光密度测定细胞浓度。培养持续了20天。在生长结束时，收集生物质并用于测量碳水化合物，蛋白质和酶促水解，以确定由于EP的存在可能引起的变化。通常，微藻可耐受高达100 mg L ^-1的扑热息痛，乙酰水杨酸和咖啡因，30 mg L ^-1的地西epa和1 mg L ^-1的氟西汀。斜生栅藻是最抗性菌株，随后C. homosphaera和螺旋藻LEB-52的抵抗力较弱。乙酰水杨酸和咖啡因影响生物质中碳水化合物和蛋白质的含量，对于白僵菌LEB-52的碳水化合物含量达到35％以上，而对斜纹葡萄球菌的蛋白含量达到66％以上。EP在酶促水解过程中没有影响。微藻对EP浓度具有抵抗力，因此成为有前途的废水生物修复技术。这些高含量的碳水化合物具有很大的价值，因为它们可用于生产生物乙醇。