Mume Fructus (MF) contains a variety of organic acids, free amino acids, and nucleoside components, and studies have not yet analyzed the relationship between the components of free amino acids and nucleosides with the varieties of MF. A rapid and sensitive method was established for simultaneous determination of 21 free amino acids and 9 nucleosides in MF by ultrafast liquid chromatography-mass spectrometry. The analysis was carried out on a Waters XBridge Amide column (100 mm × 2.1 mm, 3.5 μm) with elution by the mobile phase of 0.2% aqueous formic acid (A) and 0.2% formic acid acetonitrile (B) at a flow rate of 0.2 mL/min with 1 μL per injection. The column temperature was maintained at 30°C. The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode. The comprehensive evaluation of the samples was carried out by principal component analysis (PCA) and technique for order preference by similarity to an ideal solution (TOPSIS) analysis. Results showed the method could simultaneously determine 30 components in MF. The content of total analytes in six mainstream varieties was different, exhibited the order Nangao > Daqingmei > Zhaoshuimei > Yanmei > Shishengme > Baimei, and aspartic acid and adenosine were the most abundant amino acid and nucleoside. PCA and OPLS-DA could easily distinguish the samples, and 11 components could be chemical markers of sample classification. TOPSIS implied that the quality of Nangao and Daqingmei was superior to the other varieties. The results could provide a reliable basis for quality evaluation and utilisation of medicinal and edible MF.

中文翻译：

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基于同时测定和多元统计分析的梅子不同品种游离氨基酸和核苷的比较分析。

梅子（MF）包含多种有机酸，游离氨基酸和核苷成分，并且尚未分析游离氨基酸和核苷的成分与MF种类之间的关系。建立了一种快速灵敏的方法，通过超快速液相色谱-质谱法同时测定MF中的21个游离氨基酸和9个核苷。该分析进行上有Waters XBridge酰胺柱（100毫米×2.1毫米，3.5  μ用洗脱通过的0.2％甲酸水溶液（A）和0.2％甲酸的乙腈（B）的流动相的流率M） 0.2μ/ min和 1μ每次注射L。柱温保持在30℃。通过正离子多反应监测（MRM）模式分析目标化合物。通过主成分分析（PCA）和与理想解决方案（TOPSIS）分析相似的顺序偏好技术对样品进行了全面评估。结果表明该方法可同时测定MF中的30种成分。6个主要品种的总分析物含量不同，表现为南高>大庆梅>昭水梅>艳梅>石生梅>白梅，其中天冬氨酸和腺苷是氨基酸和核苷中含量最高的。PCA和OPLS-DA可以轻松地区分样品，其中11个成分可以作为样品分类的化学标记。TOPSIS暗示南高和大青梅的品质优于其他品种。该结果可为药用和食用MF的质量评估和利用提供可靠的依据。