Mume Fructus (MF) contains a variety of organic acids, free amino acids, and nucleoside components, and studies have not yet analyzed the relationship between the components of free amino acids and nucleosides with the varieties of MF. A rapid and sensitive method was established for simultaneous determination of 21 free amino acids and 9 nucleosides in MF by ultrafast liquid chromatography-mass spectrometry. The analysis was carried out on a Waters XBridge Amide column (100 mm × 2.1 mm, 3.5 μm) with elution by the mobile phase of 0.2% aqueous formic acid (A) and 0.2% formic acid acetonitrile (B) at a flow rate of 0.2 mL/min with 1 μL per injection. The column temperature was maintained at 30°C. The target compounds were analyzed by the positive ion multiple reaction monitoring (MRM) mode. The comprehensive evaluation of the samples was carried out by principal component analysis (PCA) and technique for order preference by similarity to an ideal solution (TOPSIS) analysis. Results showed the method could simultaneously determine 30 components in MF. The content of total analytes in six mainstream varieties was different, exhibited the order Nangao > Daqingmei > Zhaoshuimei > Yanmei > Shishengme > Baimei, and aspartic acid and adenosine were the most abundant amino acid and nucleoside. PCA and OPLS-DA could easily distinguish the samples, and 11 components could be chemical markers of sample classification. TOPSIS implied that the quality of Nangao and Daqingmei was superior to the other varieties. The results could provide a reliable basis for quality evaluation and utilisation of medicinal and edible MF.

中文翻译：

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基于同时测定和多元统计分析的不同品种乌梅中游离氨基酸和核苷的比较分析。


乌梅(MF)中含有多种有机酸、游离氨基酸和核苷成分，目前研究尚未分析游离氨基酸和核苷成分与MF品种的关系。建立了一种快速、灵敏的超快液相色谱-质谱同时测定MF中21种游离氨基酸和9种核苷的方法。分析采用 Waters XBridge Amide 柱（100 mm × 2.1 mm，3.5 μm ），流动相为 0.2% 甲酸水溶液 (A) 和 0.2% 甲酸乙腈 (B)，流速为0.2 mL/min，每次注射 1 μL 。柱温维持在30℃。采用正离子多反应监测（MRM）模式对目标化合物进行分析。采用主成分分析（PCA）和理想解相似排序技术（TOPSIS）分析对样品进行综合评价。结果表明，该方法可同时测定MF中30种成分。 6个主流品种总分析物含量不同，表现为南高>大青梅>朝水梅>艳梅>石生梅>白梅，其中天冬氨酸和腺苷含量最多。 PCA和OPLS-DA可以轻松区分样品，11种成分可以作为样品分类的化学标记。 TOPSIS表明南高和大青梅的品质优于其他品种。研究结果可为药用和食用MF的质量评价和利用提供可靠的依据。