This study was designed to test the fertilizing ability of cryopreserved turkey semen, and here, two experiments were performed: an in vitro analysis to assess the effects of Tselutin and Lake diluents and an in vivo test to determine the fertility and hatching rates by also studying the feat of three insemination doses (250, 400 and 600 × 10⁶ sperm/hen). Pooled semen samples were diluted with Tselutin or Lake extender which contained 20% of dimethylsulfoxide and 1 mM of Ficoll at final sperm concentration of 3 × 10⁹ sperm/mL. Thereafter, semen was packaged into straws and frozen on liquid nitrogen. The post-thaw sperm quality was evaluated considering motility (computer-aided sperm analysis—CASA system) and membrane integrity (flow cytometry). Significantly higher values of progressive motility and some kinetic parameters in semen frozen with Lake were found. When we compared the extenders in vivo, no significant effects were detected, whilst sperm concentration significantly affected both fertility and hatching rates, with the best results obtained with the sperm concentration of 400 × 10⁶ sperm/hen. From the results obtained, it emerged that the extender type only affected sperm motility characteristics, not the fertilizing ability of frozen-thawed semen, while inseminating dose markedly affected fertility and hatching rates.

中文翻译：

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通过评估两种稀释剂和授精剂量对土耳其精液冷​​冻保存的验证。

本研究旨在测试冷冻保存的火鸡精液的受精能力，在此进行了两个实验：通过体外分析评估Tselutin和Lake稀释剂的作用，以及通过体内研究确定生育力和孵化率的方法三种受精剂量（250、400和600×10 ⁶精子/母鸡）的壮举。浓缩的精液样品用Tselutin或Lake增量剂稀释，其中精子的终浓度为3×10 ^9，其中含有20％的二甲基亚砜和1 mM的Ficoll。精子/ mL。此后，将精液包装到吸管中并在液氮上冷冻。考虑运动性（计算机辅助精子分析-CASA系统）和膜完整性（流式细胞仪）评估解冻后的精子质量。在用Lake冷冻的精液中发现了较高的进行性运动值和一些动力学参数。当我们比较体内的补充剂时，没有发现明显的影响，而精子浓度显着影响生育力和孵化率，精子浓度为400×10 ^6时可获得最佳结果精子/母鸡 从获得的结果可以看出，增量剂类型仅影响精子的运动特性，而不影响冷冻解冻精液的受精能力，而授精剂量则显着影响生育能力和孵化率。