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Evaluation of hydrolytic enzyme activities from digestive fluids of Anthonomus grandis (Coleoptera: Curculionidae).
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-08-01 , DOI: 10.1002/arch.21730 Emiliano Ben Guerrero 1 , Ricardo Salvador 2 , Paola Talia 1
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2020-08-01 , DOI: 10.1002/arch.21730 Emiliano Ben Guerrero 1 , Ricardo Salvador 2 , Paola Talia 1
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The cotton boll weevil, Anthonomus grandis, is a major pest of cotton crops in South America. In this work, partial biochemical characterizations of (hemi) cellulases and pectinases activities in the digestive system (head‐ and gut‐ extracts) of A. grandis were evaluated. Gut extract section from third instar larvae exhibited endoglucanase, xylanase, β‐glucosidase, and pectinase activities. The endoglucanase and xylanase activities were localized in the foregut, whereas β‐glucosidase activity was mainly detected in the hindgut. In addition, no difference in pectinase activity was observed across the gut sections. Thus, A. grandis digestive system is a potentially interesting reservoir for further lignocellulolytic enzymes research.
中文翻译：
评估大按蚊消化液中的水解酶活性（鞘翅目：Curculionidae）。
棉铃象鼻虫Anthonomus grandis是南美棉花作物的主要害虫。在这项工作中，（半）纤维素酶和果胶酶的活动中的消化系统（头部和肠-提取物）局部生化表征A.巨桉进行评价。第三龄期幼虫的肠提取物切片具有内切葡聚糖酶，木聚糖酶，β-葡萄糖苷酶和果胶酶活性。内切葡聚糖酶和木聚糖酶活性位于前肠中，而β-葡萄糖苷酶活性主要在后肠中检测到。另外，在整个肠段中未观察到果胶酶活性的差异。因此，A。grandis消化系统是用于进一步木质纤维素分解酶研究的潜在有趣的储存库。
更新日期：2020-08-01
中文翻译：
评估大按蚊消化液中的水解酶活性（鞘翅目：Curculionidae）。
棉铃象鼻虫Anthonomus grandis是南美棉花作物的主要害虫。在这项工作中，（半）纤维素酶和果胶酶的活动中的消化系统（头部和肠-提取物）局部生化表征A.巨桉进行评价。第三龄期幼虫的肠提取物切片具有内切葡聚糖酶，木聚糖酶，β-葡萄糖苷酶和果胶酶活性。内切葡聚糖酶和木聚糖酶活性位于前肠中，而β-葡萄糖苷酶活性主要在后肠中检测到。另外，在整个肠段中未观察到果胶酶活性的差异。因此，A。grandis消化系统是用于进一步木质纤维素分解酶研究的潜在有趣的储存库。
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