The introduction of cryo‐techniques to the focused ion‐beam scanning electron microscope (FIB‐SEM) has brought new opportunities to study frozen, hydrated samples from the field of Life Sciences. Cryo‐techniques have long been employed in electron microscopy. Thin electron transparent sections are produced by cryo‐ultramicrotomy for observation in a cryo‐transmission electron microscope (TEM). Cryo‐TEM is presently reaching the imaging of macromolecular structures. In parallel, cryo‐fractured surfaces from bulk materials have been investigated by cryo‐SEM.

中文翻译：

---

冷冻水合生命科学样品的cryo-FIB-SEM横截面简介

将冷冻技术引入聚焦离子束扫描电子显微镜 (FIB-SEM) 为研究生命科学领域的冷冻水合样品带来了新的机会。低温技术长期以来一直用于电子显微镜。薄的电子透明切片是通过低温超薄切片术产生的，用于在低温透射电子显微镜 (TEM) 中观察。Cryo-TEM 目前正在实现大分子结构的成像。同时，通过低温扫描电镜研究了散装材料的低温断裂表面。