In an aim to prove the efficiency of polyphenols of Rosa canina fruits in promoting human health. A methanolic extract of R. canina fruits was prepared by successive maceration with solvents of increasing polarity. The polyphenol composition was analyzed by HPLC–DAD–ESI–MS. The biological activity of this extract on SH‐SY5Y cells and HepG2 cells was then studied. The antioxidant activity was tested by various in vitro tests such as DPPH‐radical‐scavenging activity, FRAP assay, hydroxyl radical scavenging assay and total antioxidant capacity. The subacute toxicity of R. canina was tested on female rats by repeated intraperitoneal administration of various doses. The phenolic profiles showed 25 antioxidants distributed into three classes of phenolic compounds: glycosylated and agglomerated flavonoids/isoflavonoids, tannins and phenanthrenes. Qualitative phytochemical analyses showed that this extract lacks alkaloids. The methanolic extract of R. canina fruits has a total antioxidant capacity of 82.69 ± 1.18 μg EAA/mg of methanol extract and the IC₅₀ of the methods used is in the following increasing order: FRAP assay (61.88 μg/ml), then hydroxyl radical scavenging assay (67.45 μg/ml) and then DPPH radical‐scavenging activity (129.81 μg/ml). The extract of R. canina did not cause any phenotypic signs of toxicity or mortality during and after treatment. The LD₅₀ was >5,000 mg/kg, hence, R. canina was considered nontoxic. An in vivo study proved the protective effect of R. canina against cardiac and hepato‐renal toxicities. These results drew the importance of a healthy diet, where diets rich in R. canina fruits can be used as a rich natural source of antioxidants and anticarcinogenic phenolic compounds.

中文翻译：

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LC-DAD / ESI-MS / MS表征玫瑰蔷薇中的酚类成分及其在细胞中的保护作用。

在一个目的是证明多酚类物质的效率的 狗蔷薇果实在促进人类健康。通过用极性增加的溶剂连续浸渍来制备加拿大猕猴桃果实的甲醇提取物。用HPLC-DAD-ESI-MS分析多酚的成分。然后研究了该提取物对SH‐SY5Y细胞和HepG2细胞的生物学活性。抗氧化剂活性通过各种体外测试进行测试，例如DPPH自由基清除活性，FRAP测定，羟基自由基清除测定和总抗氧化剂容量。狗R.亚急性毒性通过重复腹膜内施用各种剂量对雌性大鼠进行了测试。酚类谱显示25种抗氧化剂分布在三类酚类化合物中：糖基化和团聚的类黄酮/异类黄酮，单宁和菲。定性植物化学分析表明，该提取物缺乏生物碱。的甲醇提取物R.卡尼娜水果具有82.69±1.18微克EAA /甲醇提取物的毫克和IC总的抗氧化能力₅₀所使用的方法是在下面的递增顺序：FRAP测定（61.88微克/毫升），然后羟基自由基清除测定（67.45μg/ ml），然后进行DPPH自由基清除活性（129.81μg/ ml）。犬毛提取物在治疗期间和治疗后均未引起任何毒性或死亡的表型征象。LD ₅₀ > 5,000 mg / kg，因此，犬红斑病被认为是无毒的。一项体内研究证明，犬新支气管对心脏和肝肾毒性具有保护作用。这些结果吸引了健康饮食的重要性，在这种饮食中，富含R. canina水果的饮食可用作抗氧化剂和抗癌酚类化合物的丰富天然来源。