This study aimed to investigate the long-term scar hypertrophy in the rabbit transplanted oral mucosa and scrotal skin with changed matrix environment, as well as the scar location expression, quantitative analysis of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) and biomechanical changes in the transplanted tissues. The split-thickness skin grafts were collected from the oral mucosas and scrotal skins of 30 male rabbits, and prepared into reelpipes for autologous transplantation into the rabbit back muscular tissues. Samples were collected to carry out elastic tensile mechanical detection and histological observation. The maximum longitudinal tensile displacement of scrotal skin before 8 weeks of transplantation was greater than that after 8 weeks of transplantation (P < 0.05). The expression intensities of MMP-2 and TIMP-2 in the oral mucosa and in scrotal skin at 2 W time point were higher than those at T_o time point (P < 0.05). The expression quantities of TIMP-2 in oral mucosa and scrotal skin during 8–24 W were higher than those of MMP-2 (P < 0.05). At 8 W time point, the TIMP-2/MMP-2 ratio in scrotal skin was higher than that in oral mucosa (P < 0.05). MMP-2 and TIMP-2 expression in normal oral mucosa and scrotal skin is weak, but their expression is remarkably up-regulated after 2 weeks of transplantation, revealing that scar formation was related to the high expression of MMP-2 and TIMP-2. At the 8th–24th weeks, the AOD values of TIMP-2 in oral mucosa and scrotal skin are apparently higher than those of MMP-2; moreover, the TIMP-2/MMP-2 ratio in scrotal skin at the 8th week was higher than that in oral mucosa, which can well explain the earlier scar formation in scrotal skin than in oral mucosa, and it also suggests that the different expression levels between TIMP-2 and MMP-2 may account for the important cause of scar formation.

本研究旨在探讨基质环境改变的家兔口腔粘膜和阴囊皮肤移植术后长期瘢痕肥大以及瘢痕位置的表达，基质金属蛋白酶-2（MMP-2）和金属蛋白酶组织抑制剂的定量分析。 -2（TIMP-2）和移植组织的生物力学变化。从30只雄性兔子的口腔粘膜和阴囊皮肤上收集厚薄的皮肤移植物，并将其制成卷管，以自体移植到兔背部的肌肉组织中。收集样品以进行弹性拉伸机械检测和组织学观察。阴囊皮肤移植前8周的最大纵向拉伸位移大于移植8周后的最大纵向拉伸位移（P <0.05）。_Ø时间点（P <0.05）。在8-24 W期间，口腔粘膜和阴囊皮肤中TIMP-2的表达量高于MMP-2（P <0.05）。在8 W的时间点，阴囊皮肤的TIMP-2 / MMP-2比值高于口腔粘膜（P <0.05）。正常口腔粘膜和阴囊皮肤中MMP-2和TIMP-2的表达较弱，但在移植2周后它们的表达明显上调，表明瘢痕形成与MMP-2和TIMP-2的高表达有关。在第8-24周，口腔粘膜和阴囊皮肤中TIMP-2的AOD值明显高于MMP-2。此外，阴囊皮肤第8周的TIMP-2 / MMP-2比值高于口腔粘膜，这可以很好地解释阴囊皮肤比口腔粘膜更早形成疤痕的原因，