Boophone disticha (B. disticha) is a bulbous tropical and subtropical flowering plant widespread in Africa, which is frequently used to treat several human ailments. Until the present, there is no scientific validation on the biological activity of this plant from the Eastern Cape Province of South Africa and as a result, this study aimed to assess the bioactive compounds, free radicals scavenging and anticancer potentials of crude bulb extracts (chloroform, acetone, and ethanol) of Boophone disticha obtained from this geographical location. Standard biochemical techniques and Gas-chromatography mass spectrometry (GCMS) analysis were used to pinpoint the bioactive compounds in the crude extracts sequel to their antioxidant potentials against radicals such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydrogen peroxide and nitric oxide as well as their ferric ion reducing power. In addition, their cytotoxicity effects against Human cervix adenocarcinoma (HeLa) cells were assessed as an in vitro model for anticancer. The phytochemical evaluation of the crude extracts showed the presence of phenolics, flavonoids, and alkaloids. GCMS profiles confirmed the presence of some bioactive compounds in the crude extracts of B. disticha that could be responsible for their biological activities. The plant extracts possessed considerable antioxidant activity and exhibited dose-dependent radicals’ inhibition from all assays carried out. Furthermore, the cytotoxicity effects against HeLa cells recorded inhibition concentration (IC₅₀) of 1.5, 1.6, and 1.9 µg/mL for acetone, chloroform, and ethanolic extracts of B. disticha, respectively. Findings from the present study suggest that B. disticha could be a good prospective source of antioxidant and anticancer agents. Therefore, further research on the isolation and purification of compounds from these extracts are indispensable.

Boophone disticha（B. disticha）是一种鳞茎状的热带和亚热带开花植物，分布于非洲，经常用于治疗多种人类疾病。到目前为止，尚无来自南非东开普省的关于该植物生物学活性的科学证实，因此，本研究旨在评估粗鳞茎提取物（氯仿）的生物活性化合物，自由基清除能力和抗癌潜力。 ，丙酮和乙醇）Boophone disticha从该地理位置获得。使用标准生化技术和气相色谱质谱（GCMS）分析来确定粗提取物续集中的生物活性化合物对2,2-二苯基-1-吡啶并肼基（DPPH），2,2'等自由基的抗氧化能力-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS），过氧化氢和一氧化氮及其三价铁离子还原能力。另外，将它们对人宫颈腺癌细胞（HeLa）的细胞毒性作用评估为抗癌的体外模型。粗提物的植物化学评价表明存在酚类，类黄酮和生物碱。GCMS谱图证实了B. disticha粗提物中存在某些生物活性化合物可能负责他们的生物活动。从所有进行的测定中，植物提取物具有相当大的抗氧化活性，并表现出剂量依赖性自由基的抑制作用。此外，对HeLa细胞的细胞毒性作用记录为B. disticha的丙酮，氯仿和乙醇提取物的抑制浓度（IC ₅₀）分别为1.5、1.6和1.9 µg / mL 。从本研究中发现，B。disticha可能是抗氧化剂和抗癌药的良好潜在来源。因此，从这些提取物中分离和纯化化合物的进一步研究是必不可少的。