The transmission of antimicrobial resistance genes from enteric bacteria from the animal reservoir to indigenous bacteria in meat is a serious concern, as it can contribute to human exposure to antimicrobial resistance genes. The aim of this study was to investigate plasmid-mediated horizontal transfer of antimicrobial resistance genes from Escherichia coli to indigenous environmental bacteria in minced pork stored at 10 and 37 °C. E. coli MG1555 containing a gfp-tagged plasmid carrying tetracycline, kanamycin and streptomycin resistance genes was used as the donor with the indigenous bacteria in minced pork acting as potential recipients. The results demonstrated that enteric members of the pork meat microbiota were able to receive gfp-plasmids from the E. coli donor strain at both 10 and 37 °C. The majority of transconjugants were identified as Serratia spp. through sequencing of their 16S rRNA genes. This indicates that environmental Serratia spp. and other Enterobacteriaceae may play a role as carrier of antimicrobial resistance genes through the meat production chain to the consumer.

抗菌素耐药基因从肠道细菌到动物水库中的转移到肉中的本地细菌是一个令人严重关注的问题，因为它可能导致人类接触到抗菌素耐药基因。这项研究的目的是调查质粒介导的抗药性基因从大肠埃希氏菌水平转移到10和37°C储存的碎猪肉中的本地环境细菌。含有带有四环素，卡那霉素和链霉素抗性基因的带有gfp标签的质粒的大肠杆菌MG1555被用作供体，而切碎的猪肉中的本地细菌则是潜在的受体。结果表明，猪肉菌群的肠成员能够接受gfp来自大肠杆菌供体菌株的质粒在10和37°C下均存在。多数转导结合体被鉴定为沙雷氏菌。通过测序他们的16S rRNA基因。这表明环境沙雷氏菌属。肠杆菌科和其他肠杆菌科可能通过肉类生产链向消费者发挥抗药性基因的载体的作用。