Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus, its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.

猪Kobuvirus（PKV）感染在全世界的猪中非常普遍。由于从未在塞尔维亚进行过调查，因此有助于增加猪科布病毒的知识，其作用和分布，我们从家猪和野猪中测试了200个样品。从家猪中测试了10份粪便，22份脾脏和68份血清样品，以及100份来自野猪的脾脏样品。通过实时RT-PCR确定的家猪的病毒流行率为22％，野猪为6％。对3D区域进行的系统发育分析表明，从2011年开始，塞尔维亚毒株与来自野猪的匈牙利毒株关系最密切。这是塞尔维亚关于家猪和野猪中PKV的首次报道，表明其广泛传播。尽管感染与任何临床表现均无直接关系，但在粪便中发现的病毒频率表明病毒对胃肠道具有亲和力。但是，由于PKV普遍存在，