Abstract An efficient recycling method was used to develop the continuous production of high purity isomalto-oligosaccharides (IMOs) by cell associated transglucosidase of a novel strain, D. hansenii from maltose and selective fermentation by S. cerevisiae. The most potent transglucosidase producer was screened, isolated and identified as Debaryomyces hansenii using LSU region sequencing. Parameters optimization studies were investigated using whole cells of D. hansenii (∼4023 units L−1 α-glucosidase activity) from 10 L fermenter to increase the transglucosidase activity through biotransformation. IMOs was continuously synthesized by reusing the cell biomass (6 %) in a 3 L bioreactor using microfiltration membrane system with 30 % maltose concentration under controlled temperature of 34 °C in an average of 12 h for 5 cycles. The obtained low purity IMOs (67 %) was further incubated with cell pellet of isolated strain Saccharomyces cerevisiae (4 %, w/v) in 3 L bioreactor for 1 h to utilize glucose completely without affecting the product to obtain high purity IMOs by recycling method. This novel study using these yeasts, was found to utilize more than 98 % maltose with higher conversion efficiency for production of IMOs with >91 % purity, 79 % yield and highest productivity of 198.79 g L-1.h which was confirmed by HPLC.

中文翻译：

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通过分离菌株汉森德巴利酵母 SCY204 的细胞相关转葡糖苷酶生产高纯度益生元异麦芽低聚糖和酿酒酵母 SYI065 选择性发酵

摘要 采用一种高效的回收方法，通过来自麦芽糖的新型菌株汉森酵母的细胞相关转葡糖苷酶和酿酒酵母选择性发酵，开发了高纯度异麦芽低聚糖 (IMO) 的连续生产。使用 LSU 区域测序筛选、分离和鉴定最有效的转葡糖苷酶生产者为汉森德巴利酵母。参数优化研究使用来自 10 L 发酵罐的 D. hansenii 全细胞（~4023 单位 L-1 α-葡萄糖苷酶活性）进行研究，以通过生物转化增加转葡萄糖苷酶活性。通过在 3 L 生物反应器中使用具有 30% 麦芽糖浓度的微滤膜系统在 34°C 的受控温度下重复使用细胞生物质 (6%) 来连续合成 IMO，平均 12 小时持续 5 个循环。将获得的低纯度 IMO (67 %) 与分离菌株酿酒酵母 (4 %, w/v) 的细胞沉淀物在 3 L 生物反应器中进一步孵育 1 小时，以完全利用葡萄糖而不影响产品，通过回收获得高纯度 IMO方法。发现这项使用这些酵母的新研究使用了 98% 以上的麦芽糖，转化效率更高，用于生产纯度 >91%、产率 79% 和 198.79 g L-1.h 的最高生产率的 IMO，这经 HPLC 证实。