Coriander, Coriandrum Sativum L., is one of the commonest food and medicinal plants in many countries, but its chemical ingredients and pivotal role in anti-inflammatory activity have not been fully explored. The present study aimed to identify new compounds in the fruits of coriander and explore their anti-inflammatory activity. The compounds were isolated by chromatographic seperations and identified using spectroscopic and spectrometric methods. RAW264.7 macrophage cells were used to detect the anti-inflammatory activity of the compounds via Griess assay, western blotting, ELISA, and flow cytometry methods. The study resulted in the discovery of four new compounds, which were identified as: 4α-(furo[2,3-d]pyrimidin-6′-ylmethyl)-9α-propylnonolactone (1), 4-(formyloxy)-4-(6′-methylcyclohex-1-en-1-yl)butanoate(2), (7α,8α)-3α-hydroxyl-12,13α-dimethyl-5(6)-en-bicyclo[5,3,0]caprolactone (3), 7-methoxy-4-methyl-5,6-dihydro-7H-(2-hydroxypropan-2-yl)furo[2,3-f] coumarin (4). Compound 3 showed the highest anti-inflammatory activity with IC₅₀ of 6.25 μM for an inhibitory effect on nitrite oxide (NO) level. In addition, compound 3 decreased the lipopolysaccharides-stimulated generations of ROS and the inflammatory cytokines (IL-6 and TNF-α). Mechanism exploration indicated that compound 3 suppressed inflammatory mediators’ expression, like iNOS and COX-2. Furthermore, the NF-κB and MAPK pathways were involved in the anti-inflammatory process of compound 3.

在许多国家，香菜（Coriandrum Sativum L.）是最常见的食品和药用植物之一，但其化学成分及其在抗炎活性中的关键作用尚未得到充分研究。本研究旨在鉴定香菜果实中的新化合物并探索其抗炎活性。通过色谱分离法分离化合物，并使用光谱和光谱法鉴定。RAW264.7巨噬细胞用于通过Griess分析，蛋白质印迹，ELISA和流式细胞仪方法检测化合物的抗炎活性。该研究导致发现了四个新化合物，它们被鉴定为：4α-（呋喃[2,3-d]嘧啶-6'-基甲基）-9α-丙基壬内酯（1），4-（甲酰氧基）-4-（6'-甲基环己-1-烯-1-基）丁酸酯（2），（7α，8α）-3α-羟基-12,13α-二甲基-5（6）-双环[5,3,0]己内酯（3），7-甲氧基-4-甲基-5,6-二氢-7 H-（2-羟基丙烷-2-基）呋喃[2,3-f]香豆素（4）。化合物3具有最高的抗炎活性，对亚硝酸盐（NO）水平具有抑制作用，IC ₅₀为6.25μM。另外，化合物3减少了脂多糖刺激的ROS的产生和炎性细胞因子（IL-6和TNF-α）。机理研究表明，化合物3抑制炎症介质的表达，如iNOS和COX-2。此外，NF-κB和MAPK途径参与了化合物3的抗炎过程。