Phaeodactylum tricornutum was cultivated mixotrophically in batch mode providing glycerol as the C source, i.e., 0.02, 0.03 and 0.04 Mol L⁻¹ glycerol, and ultrafiltered digestate (UF) as an N source. Biomass productivity, biomass composition, N efficiency use and total energy balance were recorded and compared to those under autotrophic conditions. Under mixotrophic conditions (0.03 Mol L^-1 and 0.04 Mol L^-1 glycerol), biomass productivity of P. tricornutum increased by 1.29 and 1.60 times in comparison with autotrophic conditions.

Algal protein content declined as glycerol concentration increased, contrary to the case of the carbohydrate content. Lipid content did not change but unexpectedly, a lower unsaturated fatty acid in mixotrophic culture was observed than that from autotrophic culture. Mixotrophic conditions offered a higher energy recovery efficiency (EF_t) than autotrophic conditions (5.7 % in 0.04 Mol L⁻¹ glycerol and 4.2 % in autotrophic trial, respectively). Additionally, the efficiency of glycerol conversion into biomass (EF_gly) increased with the glycerol dose, achieving 22.8 % for 0.04 Mol L⁻¹ glycerol.

三角褐指藻以分批模式混合营养培养，提供甘油作为 C 源，即 0.02、0.03 和 0.04 Mol L ^-1甘油，以及作为 N 源的超滤消化物 (UF)。记录生物质生产力、生物质组成、氮效率使用和总能量平衡，并与自养条件下的那些进行比较。在混合营养条件下（0.03 Mol L ^-1和0.04 Mol L ^-1甘油），三角褐指藻的生物量生产力比自养条件增加了1.29和1.60倍。

与碳水化合物含量的情况相反，藻类蛋白质含量随着甘油浓度的增加而下降。脂质含量没有变化，但出乎意料的是，在混合营养培养中观察到的不饱和脂肪酸低于自养培养中的不饱和脂肪酸。混合营养条件提供了比自养条件更高的能量回收效率 (EF _t )（在 0.04 Mol L ^-1甘油中分别为5.7%和在自养试验中为 4.2%）。此外，甘油转化为生物质 (EF _gly )的效率随着甘油剂量的增加而增加，0.04 Mol L ^-1甘油达到 22.8% 。