The mitochondrial genome (mitogenome) can help us understand the phylogenetic relationships within the genus Lethe and the subfamily Satyrinae. In this study, we sequenced the complete mitogenomes of 14 Lethe species, which range in size from 15,225 to 15,271 bp, with both 37 genes (13 PCGs, 22 tRNAs, 2 rRNAs) and a noncoding A + T-rich region. The gene arrangement and orientation is similar to typical mitogenomes of Lepidoptera. The Ka/Ks ratio shows that cox1 has the slowest evolutionary rate. The secondary structure of trnN lacks the Pseudouracil loop (TψC loop) in most Lethe species. The inferred phylogenetic analyses show that Lethe is a well-supported monophyletic group, and reveal 2 major clades within the genus Lethe, which is consistent with previous morphological classifications.

线粒体基因组（mitogenome）可以帮助我们了解Lethe属和 Satyrinae 亚科内的系统发育关系。在这项研究中，我们对 14 个Lethe物种的完整有丝分裂基因组进行了测序，这些物种的大小范围从 15,225 到 15,271 bp，包括 37 个基因（13 个 PCG、22 个 tRNA、2 个 rRNA）和一个非编码 A+T 丰富的区域。基因排列和方向与典型的鳞翅目有丝分裂基因组相似。Ka/Ks 比率表明cox1的进化速度最慢。在大多数Lethe物种中，trnN 的二级结构缺乏假尿嘧啶环（TψC 环）。推断的系统发育分析表明Lethe是一个得到充分支持的单系群，并揭示了Lethe属内的 2 个主要进化枝，这与以前的形态分类一致。