Recently, we have reported trained innate immunity in laying chicken monocytes. In the present study, we further investigated trained innate immunity of monocytes in layers and broilers. Monocytes of both breeds isolated from blood were trained in vitro with β-glucan, rec-chicken IL-4 or a combination of both, and restimulated with lipopolysaccharide (LPS), after which inflammation and metabolism-related responses were measured. Training of laying and broiler hen monocytes resulted in increased mRNA levels of IL-1β, iNOS and HIF-1α, but enhanced surface expression of CD40 and NO production was only observed in layers. Our in vitro study demonstrates that monocytes from different genetic backgrounds can be trained. However, the observed differences suggest a differential effect on immune functionality associated with innate training. Whether these differences in immune functions between layers and broilers have effect on disease resistance remains to be elucidated.

最近，我们报道了在产蛋鸡单核细胞中经过训练的先天免疫。在本研究中，我们进一步研究了蛋鸡和肉鸡单核细胞经过训练的先天免疫。从血液中分离出的两个品种的单核细胞在体外用 β-葡聚糖、rec-鸡 IL-4 或两者的组合进行训练，并用脂多糖 (LPS) 再刺激，然后测量炎症和代谢相关反应。蛋鸡和肉鸡单核细胞的训练导致 IL-1β、iNOS 和 HIF-1α 的 mRNA 水平增加，但仅在蛋鸡中观察到 CD40 和 NO 产生的表面表达增强。我们的体外研究表明，可以训练来自不同遗传背景的单核细胞。然而，观察到的差异表明对与先天训练相关的免疫功能有不同的影响。蛋鸡和肉鸡之间的这些免疫功能差异是否对疾病抵抗力有影响仍有待阐明。