In many aquatic species, the negative effect of temperature variations has a significant impact on physiological performance since beyond Tp (upper pejus) and Tc (critical temperatures), according to the oxygen- and capacity-limited thermal tolerance (OCLTT), transition to hypoxemia and mitochondrial metabolism triggers the increase in reactive oxygen species (ROS) production. However, climate change may have different spatial impact, and as a result, areas with more favorable climatic conditions (refugia) can be identified. The aim of the present study, based on cellular stress responses, is the demarcation of these areas and the preservation of commercially important marine species. Under this prism, individuals of the species Callinectes sapidus (blue crab), Sepia officinalis (common cuttlefish), Holothuria tubulosa (sea cucumber) and Venus verrucosa (clam) from Thermaikos, Pagasitikos and Vistonikos gulf were collected seasonally. The results showed an increase in the levels of several stress indicators exhibiting the triggering of Heat Shock Response, MAPK activation, apoptotic phenomena and increased ubiquitilination during the summer sampling in relation to the spring and autumn samplings concerning blue crab and clam, while no changes were observed for common cuttlefish and sea cucumber. It seems that these cellular responses consist a cytoprotective mechanism against environmental thermal stress. Regarding collection sites, for all examined species, higher cellular stress levels were observed in Pagasitikos, and lower in Vistonikos gulf. This analysis of biochemical and molecular markers is expected to provide a clearer picture for the definition of “refugia” for the above species.

在许多水生物种中，温度变化的负面影响会对生理性能产生重大影响，因为根据氧气和容量限制的热耐受性（OCLTT），超出Tp（上皮水）和Tc（临界温度）后，过渡为低氧血症线粒体的新陈代谢触发了活性氧（ROS）产生的增加。但是，气候变化可能具有不同的空间影响，因此，可以确定气候条件更为有利的地区（避难所）。基于细胞应激反应，本研究的目的是对这些区域的划分以及对商业上重要的海洋物种的保护。在这个棱镜下，Callinectes sapidus（蓝蟹），棕褐色的个体（常见的墨鱼），海参（海参）和维纳斯疣季节性收集来自Thermaikos，Pagasitikos和Vistonikos海湾的（蛤）。结果表明，与蓝蟹和蛤的春季和秋季采样相比，夏季采样期间与春季和秋季采样相比，表现出触发热休克反应，MAPK活化，凋亡现象和泛素化增加的几种应激指标的水平增加，而没有变化。观察常见的墨鱼和海参。似乎这些细胞反应包括针对环境热应激的细胞保护机制。关于收集地点，对于所有检查的物种，在Pagasitikos中观察到较高的细胞应激水平，在Vistonikos海湾中观察到较低的细胞应激水平。预期该生化和分子标记的分析将为上述物种的“避难所”定义提供更清晰的画面。