In this study, we investigated the improvement in biochemical parameters by adding gibberellic acid (GA₃) and salicylic acid to mixotrophy medium. Fresh weight of the cultures treated with 100 mM SA and GA₃ was approximately 30% higher than that of control cultures. Both GA₃ and SA induced chlorophyll, carotenoid, and astaxanthin accumulation in Scenedesmus. SA increased carotenoid and astaxanthin content 2 and 2.6 times more than GA₃ respectively. Protein content decreased in microalgae treated by GA₃ and SA. 100 mM concentration of GA₃ and SA caused a significant increase in lipid levels (42.6% and 41%, respectively). Compared with the controls, lower proportions of unsaturated fatty acids (USFAs) were detected in the GA₃-and SA-treated biomass. In the microalgae treated by GA_3, an insignificant decrease was detected in saturated fatty acids (SFA) and USFAs in comparison to the controls. New fatty acids, SFA C16:0 (2.5%), and USFA C22:6 (n3) (docosahexaenoic (DHA) (2.1%) were added to the fatty acid profile. Adding SA to the microalgal cultures significantly increased the amount of SFAs and reduced the amount of USFAs compared to the controls. 21.2% C8:0 and 29.4% EPA had the highest content in SFAs and USFAs, respectively. Our results showed that GA₃ and SA (especially SA) can be good candidates for improving carotenoid and astaxanthine under mixotrophy conditions and changing the amount of special fatty acids.

在这项研究中，我们研究了通过向混合营养培养基中添加赤霉素（GA ₃）和水杨酸来改善生化参数。用100 mM SA和GA ₃处理的培养物的鲜重比对照培养物的鲜重大约高30％。GA ₃和SA都诱导了景物中的叶绿素，类胡萝卜素和虾青素积累。SA使类胡萝卜素和虾青素含量分别比GA ₃增加2倍和2.6倍。GA ₃和SA处理的微藻中蛋白质含量降低。浓度为100 mM的GA ₃和SA导致血脂水平显着增加（分别为42.6％和41％）。与对照相比，在GA ₃和SA处理的生物质中检测到较低比例的不饱和脂肪酸（USFA）。与对照组相比，在用GA ₃处理的微藻中，饱和脂肪酸（SFA）和USFA的减少量很小。在脂肪酸谱中添加了新的脂肪酸SFA C16：0（2.5％）和USFA C22：6（n3）（二十二碳六烯酸（DHA）（2.1％）），在微藻培养物中添加SA显着增加了SFA的数量结果表明，GA ₃含量最高，分别为21.2％C8：0和29.4％EPA。 和SA（尤其是SA）可以在混合营养条件下改善类胡萝卜素和虾青素并改变特殊脂肪酸的量。