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Anesthesia plus surgery in neonatal period impairs preference for social novelty in mice at the juvenile age.
Biochemical and Biophysical Research Communications ( IF 2.5 ) Pub Date : 2020-08-01 , DOI: 10.1016/j.bbrc.2020.07.108
Xin Jin 1 , Lei Ji 1 , Qingcai Chen 1 , Rui Sheng 2 , Fuhai Ji 1 , Jianping Yang 1
Affiliation  

Anesthetic sevoflurane could induce neurotoxicity in developing brain and cause adverse neurobehavioral outcomes in mice, including inattention, social interaction deficit, and learning and memory impairment. However, there is less data on the effect of anesthesia plus surgery on social interaction behavior. Therefore, we investigated whether the combination of anesthesia and surgical stimulation could induce behavioral and biochemical changes in mice. Firstly, the six-day-old mice were received either 3% sevoflurane anesthesia or abdominal surgery under sevoflurane anesthesia. Then, these mice were scheduled to social interaction test in three-chambered social paradigm at one-month-old. In addition, the brain tissues of neonatal mice were harvested at 24 h after treatment, for measuring the levels of OXTR and NMDAR1 in Western blot analysis. We found that neonatal anesthesia with sevoflurane in a clinically-relevant dosage could not induce social interaction deficit. Nevertheless, anesthesia plus surgery was able to impair preference for social novelty in mice. Moreover, anesthesia plus surgery decreased the levels of OXTR in hippocampus and cortex of mice, as well as NMDAR1 in hippocampus. Collectively, these results suggested that anesthesia plus surgery could impair social novelty preference, but not sociability in mice, and that social memory might be more vulnerable than social affiliation in biological property. Furthermore, reduction in the levels of cortex OXTR and hippocampus NMDAR1 could be associated with social recognition memory in mice.



中文翻译:

新生儿时期的麻醉加手术损害了幼龄小鼠对社交新颖性的偏爱。

麻醉性七氟醚可能在发育中的大脑中引起神经毒性,并引起小鼠不良的神经行为结果,包括注意力不集中,社交互动不足以及学习和记忆障碍。但是,关于麻醉加手术对社交互动行为影响的数据较少。因此,我们调查了麻醉和手术刺激的组合是否可以诱导小鼠的行为和生化变化。首先,对六日龄小鼠进行3%的七氟醚麻醉或在七氟醚麻醉下进行腹部手术。然后,将这些小鼠安排在一个月大的三室社交范式中进行社交互动测试。另外,在处理后24小时收集新生小鼠的脑组织,以在Western印迹分析中测量OXTR和NMDAR1的水平。我们发现七氟醚与临床相关剂量的新生儿麻醉不能引起社交互动障碍。然而,麻醉加手术能够损害小鼠对社交新颖性的偏好。此外,麻醉加手术降低了小鼠海马和皮层的OXTR水平以及海马的NMDAR1水平。总体而言,这些结果表明,麻醉加手术可能会损害社会对新奇事物的偏好,但不会损害小鼠的社交能力,并且社会记忆可能比生物学上的社会隶属关系更脆弱。此外,皮质OXTR和海马NMDAR1水平的降低可能与小鼠的社会认知记忆有关。麻醉加手术能够损害小鼠对社交新颖性的偏好。此外,麻醉加手术降低了小鼠海马和皮层的OXTR水平以及海马的NMDAR1水平。总体而言,这些结果表明,麻醉加手术可能会损害社会对新奇事物的偏好,但不会损害小鼠的社交能力,并且社会记忆可能比生物学上的社会隶属关系更脆弱。此外,皮质OXTR和海马NMDAR1水平的降低可能与小鼠的社会认知记忆有关。麻醉加手术能够损害小鼠对社交新颖性的偏好。此外,麻醉加手术降低了小鼠海马和皮质的OXTR水平,以及海马的NMDAR1水平。总体而言,这些结果表明,麻醉加手术可能会损害社会对新奇事物的偏好,但不会损害小鼠的社交能力,并且社会记忆可能比生物学上的社会归属更脆弱。此外,皮质OXTR和海马NMDAR1水平的降低可能与小鼠的社会认知记忆有关。这些结果表明,麻醉加手术可能会损害社会对新奇事物的偏好,但不会损害小鼠的社交能力,并且社会记忆可能比生物学上的社会归属更脆弱。此外,皮质OXTR和海马NMDAR1水平的降低可能与小鼠的社会认知记忆有关。这些结果表明,麻醉加手术可能会损害社会对新奇事物的偏好,但不会损害小鼠的社交能力,并且社会记忆可能比生物学上的社会归属更容易受到伤害。此外,皮质OXTR和海马NMDAR1水平的降低可能与小鼠的社会认知记忆有关。

更新日期:2020-08-20
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