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A Cellulolytic Streptomyces Sp. Isolated from a Highly Oligotrophic Niche Shows Potential for Hydrolyzing Agricultural Wastes
BioEnergy Research ( IF 3.6 ) Pub Date : 2020-07-31 , DOI: 10.1007/s12155-020-10174-z
Samuel Celaya-Herrera , Luz E. Casados-Vázquez , Idania Valdez-Vazquez , Francisco Barona-Gómez , Dennis K. Bideshi , José E. Barboza-Corona

Cellulases from Streptomyces species can be used as auxiliary agents in the pretreatment of lignocellulosic materials, but little work has been done on this topic. Besides, Streptomyces strains have been identified from Cuatro Cienegas basin, but their potential in the treatment of agricultural wastes has not been demonstrated. In this work a Streptomyces sp., strain CC48 isolated from the oligotrophic environment, shows high levels of cellulolytic activity (0.76 U/ mL at pH 7.0, 60 °C) in minimum medium supplemented with carboxymethyl cellulose compared with other Streptomyces strains and isolates in a bacterial collection. At least three putative cellulases (~30, 40, and 45 kDa) were identified in Streptomyces sp. CC48 extracts. The addition of Mg2+ increased cellulase activity by 23%, whereas other cations resulted in decreased catalysis. Residual cellulolytic activities after 4 and 8 h of incubation at 60 °C were ~ 73 and 65%, respectively, and ~ 98% at 50 °C after 8 h. Corn cob and wheat straw pretreated with an alkaline method showed saccharification values of 320 and 330 mg/g of reducing sugars per gram of dry substrate, respectively. The significance of this work lies in the fact that data provided here show that Streptomyces sp. strain CC48 is capable of hydrolyzing both maize and wheat substrates, suggesting the potential of this bacterium to hydrolyze agricultural wastes. Also, Cuatro Cienegas Basin may represent a source for efficient cellulolytic microorganisms.



中文翻译:

纤维素酶链霉菌 从高度贫营养的生态位中分离出具有水解农业废物的潜力

链霉菌属物种的纤维素酶可以用作木质纤维素材料预处理中的辅助剂,但是在该主题上所做的工作很少。此外,从Cuatro Cienegas盆地中已鉴定出链霉菌菌株,但尚未证明其在农业废物处理中的潜力。在这项工作中,从贫营养环境中分离出的链霉菌属CC48菌株与其他链霉菌菌株和分离株相比,在补充了羧甲基纤维素的最低培养基中显示出高水平的纤维素分解活性(在pH 7.0,60°C,0.76 U / mL)。细菌集合。在链霉菌中至少鉴定出三种假定的纤维素酶(约30、40和45 kDa)sp。CC48提取物。Mg 2+的添加使纤维素酶活性增加了23%,而其他阳离子导致催化作用降低。在60°C孵育4和8 h后,残留的纤维素分解活性分别为〜73和65%,在50°C孵育8 h后为〜98%。碱法预处理的玉米芯和麦草显示出的糖化值为每克干基质分别为320和330 mg / g还原糖。这项工作的意义在于,此处提供的数据表明链霉菌属。CC48菌株能够水解玉米和小麦底物,表明该细菌具有水解农业废物的潜力。同样,Cuatro Cienegas盆地可能代表了高效纤维素分解微生物的来源。

更新日期:2020-08-01
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