Ferulic acid is a known precursor for vanillin production but the significance of agro waste as substrates for its extraction, in combination with microbes is a less explored option. Various lactic acid bacteria were screened for the production of ferulic acid esterase (FAE) and Enterococcus lactis SR1 was found to produce maximum FAE (7.54 ± 0.15 IU/ml) in the synthetic medium under submerged fermentation. To make the process cost effective, various lignocellulosic agroresidues were evaluated for the production of FAE from the bacterium. It was found that wheat bran serves as the best substrate for FAE production (4.18 ± 0.12 IU/ml) from E. lactis SR1. Further, optimization of fermentation conditions for FAE production from E. lactis SR1 using wheat bran as carbon source led to an increase in the enzyme production (7.09 ± 0.21 IU/ml) by 1.5 fold. The FAE produced was used alone or in combination with commercial holocellulase for biological release of FA from the tested agroresidues. The highest release of FA (mg/g) by enzymatic extraction occurred in sugarbeet pulp (2.56), followed by maize bran (1.45), wheat bran (1.39) and rice bran (0.87), when both the enzymes (FAE and holocellulase) were used together. Alkaline extraction and purification of ferulic acid (FA) from these agro residues also showed that sugarbeet pulp contains the highest amount of FA (5.5 mg/g) followed by maize bran (3.0 mg/g), wheat bran (2.8 mg/g) and rice bran (1.9 mg/g), similar to the trend obtained in biological/enzymatic extraction of FA from these residues. Furthermore, the substrates were found to release higher reducing sugars when both commercial holocellulase and FAE were used in combination than by the use of holocellulase alone. Thus, FAEs not only release FA but also enabled hemicellulase and cellulase to release more sugars from plant material.

阿魏酸是香草醛生产的已知前体，但农业废物作为其提取基质的重要性，与微生物相结合是一个较少探索的选择。筛选了各种乳酸菌以生产阿魏酸酯酶 (FAE)，发现乳酸肠球菌SR1 在深层发酵的合成培养基中产生最大的 FAE (7.54 ± 0.15 IU/ml)。为了使该过程具有成本效益，评估了各种木质纤维素农业残留物从细菌中生产 FAE 的情况。发现麦麸是E.lactis SR1生产 FAE 的最佳底物（4.18 ± 0.12 IU/ml）。此外，用于从生产FAE的发酵条件的优化E.球菌SR1 使用麦麸作为碳源导致酶产量 (7.09 ± 0.21 IU/ml) 增加 1.5 倍。产生的 FAE 单独使用或与商业全纤维素酶结合使用，用于从测试的农业残留物中生物释放 FA。酶法提取的 FA 释放量最高 (mg/g) 发生在甜菜果肉中 (2.56)，其次是玉米麸 (1.45)、麦麸 (1.39) 和米糠 (0.87)，当两种酶（FAE 和全纤维素酶）一起使用。从这些农业残留物中碱提取和纯化阿魏酸 (FA) 还表明，甜菜浆中的 FA 含量最高 (5.5 mg/g)，其次是玉米麸 (3.0 mg/g)、小麦麸 (2.8 mg/g)和米糠 (1.9 mg/g)，与从这些残留物中生物/酶法提取 FA 中获得的趋势相似。此外，与单独使用全纤维素酶相比，当商业全纤维素酶和 FAE 联合使用时，发现底物释放更高的还原糖。因此，FAEs 不仅释放 FA，而且使半纤维素酶和纤维素酶能够从植物材料中释放更多的糖。