Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits. Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization. In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler. The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts. Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler. In vitro cell adhesion, proliferation and differentiation were evaluated to determine the biocompatibility of the composites. On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticl...

中文翻译：

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使用含有睡茄提取物的羟基磷灰石陶瓷骨替代品增强骨矿化。

Withania somnifera (ashwagandha) 因其各种健康益处而被用于印度传统医学。Withaferin-A 是一种存在于这种草药中的甾体内酯，已显示出基于蛋白体抑制的骨矿化增强。在目前的工作中，壳聚糖微粒与 W. somnifera 的总甲醇根提取物混合作为致孔剂掺入基于磷酸钙的羟基磷灰石骨填料中。生物活性分子的受控释放能够增强前成骨细胞的增殖和分化。微粒百分比经过优化，对羟基磷灰石骨填料的凝固时间、机械强度和降解性的影响最小。评估体外细胞粘附、增殖和分化，以确定复合材料的生物相容性。