The plant Sterculia urens Roxb. of Malvaceae family is comparatively understudied. Genus Sterculia is widely recognized by its phytomedicinal and ethnomedicinal attributes. The study is aimed to evaluate the qualitative analysis, Fourier transform infrared (FT-IR), thin layer chromatography (TLC), total phenolic content (TPC), antioxidant and antimicrobial activities of the crude hydro-methanolic extract of S. urens root. The antioxidant activity, antimicrobial assay for clinical isolates, and TPC were measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity, agar well diffusion method, and Folin–Ciocalteu assay respectively. Hydro-methanolic extract confirmed the presence of alkaloids, flavonoids, tannins, phenols, saponins, steroids, and glycosides as primary and secondary metabolites, which was later confirmed by TLC. FT-IR spectroscopy revealed the presence of alkanes, alkenes, alkyl halides, halogen compounds, primary alcohol, tertiary alcohol, aldehyde, aromatic amine, secondary amines, amide group, and carboxylic acid. The crude extract was composed of a significant quantity of total phenolic content with 705 ± 0.40 mg GAE/g. Synergistically, the IC50 value of the crude extract and ascorbic acid was found to be 27.055 μg/ml and 37.244 μg/ml, respectively, which suggests that root extract possesses strong antioxidant properties. The majority of the microbial strains exhibited varying degrees of sensitivity to the root extract with a notable inhibitory effect against Escherichia coli, Klebsiella pneumoniae, and Penicillium glaucum. The findings of this analysis suggest that the hydro-methanolic extract from S. urens root exhibit antioxidant activity quantified by its ability to scavenge DPPH; antimicrobial activity displayed appreciable microbial sensitivity. These properties are associated with the presence of high phenolic content, different secondary metabolites, and their functional groups. The results are suggestive that S. urens root is rich in bioactive compounds, which serve as a novel natural source for potential therapeutic applications.

中文翻译：

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斯特氏菌（Sterculia urens Roxb）的体外抗氧化剂和抗菌潜力。根提取物及其生物活性植物成分评估

植物Sterculia urens Roxb。Malvaceae家族的研究相对较少。气志属植物以其植物药理和人种学特性而被广泛认可。这项研究旨在评估定性分析，S。urens根粗水-甲醇提取物的傅里叶变换红外光谱（FT-IR），薄层色谱法（TLC），总酚含量（TPC），抗氧化剂和抗菌活性。分别通过DPPH（2,2-二苯基-1-picylhydrazyl）自由基清除活性，琼脂井扩散法和Folin-Ciocalteu分析测定抗氧化活性，临床分离株的抗菌活性和TPC。氢甲醇提取物证实存在生物碱，类黄酮，单宁，酚，皂角苷，类固醇和糖苷作为主要代谢产物和次要代谢产物，后来通过TLC证实。FT-IR光谱显示存在烷烃，烯烃，卤代烷，卤素化合物，伯醇，叔醇，醛，芳族胺，仲胺，酰胺基和羧酸。粗提取物由大量的总酚含量组成，为705±0.40 mg GAE / g。协同发现，粗提物和抗坏血酸的IC50值分别为27.055μg/ ml和37.244μg/ ml，这表明根提取物具有很强的抗氧化性能。大多数微生物菌株对根提取物表现出不同程度的敏感性，并对大肠杆菌，肺炎克雷伯菌和青霉菌有明显的抑制作用。该分析的结果表明，从S.中提取了氢甲醇。ur根表现出的抗氧化活性由其清除DPPH的能力量化；抗菌活性显示出明显的微生物敏感性。这些性质与高酚含量，不同的次生代谢产物及其官能团的存在有关。结果表明，S。urens根富含生物活性化合物，可作为潜在治疗应用的新型天然来源。