The cause of the immunogenicity of Acanthopanax senticosus injection has been studied. The method used for the preparation of the injection includes boiling the plant material in water, precipitation with ethanol, and filtration to produce the extract. In the current study, this extract was treated with saturated ammonium sulfate to collect the protein from the extract. Indicators of systemic allergy, including total immunoglobulin (IgE), interleukin -4 (IL-4), and interferon-gamma (IFN-γ) levels in the antiserum of Guinea pigs sensitized with this protein extract were used to determine the immunogenicity of the protein extract. Testing was performed using Western blotting to identify IgE in antiserum binding in Guinea pigs sensitized by injection. The target protein band was further identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. The protein extract from A. senticosus caused positive reactions and significantly increased the levels of total IgE, IL-4, and IFN-γ in the serum of sensitized Guinea pigs. Western blotting showed that a group of proteins with a molecular weight of 60 000 in the A. senticosus protein extract acted as the major immunogenic protein by binding to serum IgE of Guinea pigs sensitized with A. senticosus injection. The protein was a pyruvate kinase homologous protein with high sequence similarity to the pyruvate kinase protein of Vitis vinifera. It was concluded that the allergic reaction caused by the injection of A. senticosus may be related to the interaction of these macromolecular proteins within the body.

刺五加免疫原性的原因已经研究了注射剂。用于制备注射剂的方法包括将植物材料在水中煮沸，用乙醇沉淀和过滤以产生提取物。在当前的研究中，该提取物用饱和硫酸铵处理以从提取物中收集蛋白质。用该蛋白提取物敏化的豚鼠抗血清中的全身过敏性指标，包括总免疫球蛋白（IgE），白介素-4（IL-4）和干扰素-γ（IFN-γ）水平，用于确定该蛋白提取物的免疫原性。蛋白质提取物。使用蛋白质印迹法进行测试以鉴定注射致敏的豚鼠抗血清结合中的IgE。通过基质辅助激光解吸/电离飞行时间质谱进一步鉴定目标蛋白带。蛋白质提取物假单胞菌引起阳性反应，并显着增加致敏的豚鼠血清中总IgE，IL-4和IFN-γ的水平。蛋白质印迹法显示，刺参曲霉蛋白质提取物中的一组分子量为60 000的蛋白质通过结合到用刺参曲霉注射液致敏的豚鼠的血清IgE上充当主要的免疫原性蛋白质。该蛋白是丙酮酸激酶同源蛋白，与葡萄（Vitis vinifera）的丙酮酸激酶蛋白具有很高的序列相似性。结论是，注射刺参曲霉引起的过敏反应可能与体内这些大分子蛋白质的相互作用有关。