Mitochondrial biology is underpinned by the presence and activity of large protein complexes participating in the organelle-located parts of cellular respiration, the TCA cycle and oxidative phosphorylation. While the enzymatic roles of these complexes are undisputed, little is known about the interactions of the subunits beyond their presence in the monomeric protein complexes and their functions in regulating mitochondria metabolism. By applying one of the most important regulatory cues for plant metabolism, the presence or absence of light, we here assess the changes in the composition and molecular mass of known mitochondrial protein complexes by employing a differential complexome profiling strategy. Covering a mass range up to 25 MDa, we demonstrate dynamic associations of TCA-cycle enzymes and of OXPHOS components. The data presented here form the basis for future studies aiming to advance our understanding of the role of protein:protein interactions in the regulation of plant mitochondrial functions.

中文翻译：

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拟南芥叶片线粒体中蛋白质相互作用模式对照明的响应变化。

线粒体生物学的基础是参与细胞呼吸，TCA循环和氧化磷酸化的细胞器定位部分的大蛋白复合物的存在和活性。尽管这些复合物的酶作用是毋庸置疑的，但是除了亚基在单体蛋白质复合物中的存在及其在调节线粒体代谢中的功能外，关于亚基之间的相互作用还知之甚少。通过应用植物代谢最重要的调控线索之一，即是否存在光，我们在这里通过采用差异复合物谱分析策略评估已知线粒体蛋白质复合物的组成和分子质量的变化。涵盖了高达25 MDa的质量范围，我们证明了TCA-循环酶和OXPHOS组分的动态关联。