Intrinsically Bioactive Cryogels Based on Platelet Lysate Nanocomposites for Hemostasis Applications.,Biomacromolecules

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Intrinsically Bioactive Cryogels Based on Platelet Lysate Nanocomposites for Hemostasis Applications.
Biomacromolecules ( IF 5.5 ) Pub Date : 2020-07-30 , DOI: 10.1021/acs.biomac.0c00787
Bárbara B Mendes _{1,

2} , Manuel Gómez-Florit _{1,

2} , Ana C Araújo _{1,

2} , Justina Prada ₃ , Pedro S Babo _{1,

2} , Rui M A Domingues _{1,

2} , Rui L Reis _{1,

2} , Manuela E Gomes _{1,

2}

Affiliation

The currently used hemostatic agents are highly effective in stopping hemorrhages but have a limited role in the modulation of the wound-healing environment. Herein, we propose an intrinsically bioactive hemostatic cryogel based on platelet lysate (PL) and aldehyde-functionalized cellulose nanocrystals (a-CNCs). PL has attracted great attention as an inexpensive milieu of therapeutically relevant proteins; however, its application as a hemostatic agent exhibits serious constraints (e.g., structural integrity and short shelf-life). The incorporation of a-CNCs reinforced the low-strength PL matrix by covalent cross-linking its amine groups that exhibit an elastic interconnected porous network after full cryogelation. Upon blood immersion, the PL-CNC cryogels absorbed higher volumes of blood at a faster rate than commercial hemostatic porcine gelatin sponges. Simultaneously, the cryogels released biomolecules that increased stem cell proliferation, metabolic activity, and migration as well as downregulated the expression of markers of the fibrinolytic process. In an in vivo liver defect model, PL-CNC cryogels showed similar hemostatic performance in comparison with gelatin sponges and normal material-induced tissue response upon subcutaneous implantation. Overall, owing to their structure and bioactive composition, the proposed PL-CNC cryogels provide an alternative off-the-shelf hemostatic and antibacterial biomaterial with the potential to deliver therapeutically relevant proteins in situ.

中文翻译：

基于血小板裂解物纳米复合材料的固有生物活性冰冻剂，用于止血应用。

当前使用的止血剂在止血方面非常有效，但是在调节伤口愈合环境方面作用有限。本文中，我们提出了一种基于血小板裂解物（PL）和醛官能化纤维素纳米晶体（a-CNCs）的内在生物活性止血凝胶。PL作为治疗相关蛋白的廉价环境已引起广泛关注。然而，其作为止血剂的应用存在严重的局限性（例如，结构完整性和较短的保质期）。a-CNC的掺入通过共价交联其胺基团增强了低强度PL基质，该胺基团在完全冷冻凝胶后呈现出弹性互连的多孔网络。浸入血液后，PL-CNC冷冻凝胶比商用止血猪明胶海绵吸收的血液量更快。同时，冷冻凝胶释放了生物分子，从而增加了干细胞的增殖，代谢活性和迁移，并下调了纤溶过程标志物的表达。在体内肝缺陷模型，PL-CNC冷冻凝胶与明胶海绵和皮下植入后正常材料诱发的组织反应相比，具有相似的止血性能。总体而言，由于其结构和生物活性成分，拟议的PL-CNC冷冻凝胶提供了另一种现成的止血和抗菌生物材料，具有在原位递送治疗相关蛋白的潜力。

更新日期：2020-09-14

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