Abstract

Oxidative stress and inflammation are necessary pathogenic factors contributing to the aetiology of diabetic retinopathy (DR). Triptolide (TPL) is derived from the traditional Chinese herb lei gong teng with anti-inflammatory, immunosuppressive and antitumor activities. This article was developed to examine the effect of TPL on DR. ARPE-19 cells were pre-treated with TPL and then stimulated by high glucose (HG). We found that TPL treatment enhanced cell viability, decreased apoptosis and ROS production in HG-treated RPE cells. MiR-29b was low-expressed in HG-treated cells, but TPL raised its expression. In addition, the protective activity of TPL towards ARPE-19 cells was attenuated when miR-29b was reduced. By utilising bioinformatics evaluation, PTEN was predicted as a downstream target of miR-29b. Also, TPL obstructed PI3K/AKT signalling pathways in HG-treated ARPE-19 Cells. Taken together, TPL secured ARPE-19 cells from HG-induced oxidative damage via regulating miR-29b/PTEN axis.

摘要

氧化应激和炎症是导致糖尿病视网膜病变 (DR) 病因的必要致病因素。雷公藤内酯 (TPL) 源自中药雷公藤具有抗炎、免疫抑制和抗肿瘤活性。本文旨在研究 TPL 对 DR 的影响。ARPE-19 细胞用 TPL 预处理，然后用高葡萄糖 (HG) 刺激。我们发现 TPL 处理增强了细胞活力，减少了 HG 处理的 RPE 细胞的细胞凋亡和 ROS 产生。MiR-29b 在 HG 处理的细胞中低表达，但 TPL 提高了其表达。此外，当 miR-29b 减少时，TPL 对 ARPE-19 细胞的保护活性减弱。通过利用生物信息学评估，预测 PTEN 是 miR-29b 的下游靶标。此外，TPL 阻碍了 HG 处理的 ARPE-19 细胞中的 PI3K/AKT 信号通路。总之，TPL 通过调节 miR-29b/PTEN 轴保护 ARPE-19 细胞免受 HG 诱导的氧化损伤。