Abstract Colletotrichum fructicola causes strawberry anthracnose, which results in considerable economic losses in quality and production in Asia. However, the molecular mechanism of strawberry infection with C. fructicola is still poorly understood. Here, we employed RNA-Seq technology to mine the strawberry transcriptome for EST-SSRs (Expressed Sequence Tag–Simple Sequence Repeats) following C. fructicola infection. Fortunately, we detected 3019 potential EST-SSRs from the transcriptomic data of cv. Jiuxiang. The seven commonly occurring repeat motifs were A/T (278), GAA/TTC (263), GAG/CTC (215), AAG/CTT(188), GGA/TCC (163), AGA/TCT (153) and AGG/CCT (153). We identified 31, 84 and 129 differentially expressed EST-SSR-containing sequences corresponding to 24, 72 and 96 Hpi, while 13 DE-EST-SSRs -containing sequences were differentially expressed and common for all the three time points. Furthermore, we identified allele polymorphisms for some DE-EST-SSRs among several resistant and susceptible strawberry varieties and wild species. The EST-SSRs in our research were related to the reported QTL resistance loci in strawberry which are differentially expressed after C. fructicola infection and have allele polymorphisms in different strawberry varieties and wild species. These provide a clue for utilisation of the EST-SSRs in marker-assisted breeding to improve anthracnose-resistant breeding in future.

中文翻译：

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草莓（Fragaria ×ananassa Duchase）与果炭疽菌感染相关的Est-SSR标记的表征

摘要 果炭疽菌引起草莓炭疽病，给亚洲地区的草莓质量和生产造成了相当大的经济损失。然而，草莓感染 C. fructicola 的分子机制仍知之甚少。在这里，我们采用 RNA-Seq 技术来挖掘草莓转录组的 EST-SSR（表达序列标签-简单序列重复）。幸运的是，我们从 cv 的转录组数据中检测到 3019 个潜在的 EST-SSR。九乡。七个常见的重复基序是 A/T (278)、GAA/TTC (263)、GAG/CTC (215)、AAG/CTT(188)、GGA/TCC (163)、AGA/TCT (153) 和 AGG /CCT (153)。我们鉴定了 31、84 和 129 个差异表达的含 EST-SSR 序列，分别对应于 24、72 和 96 Hpi，而 13 个含有 DE-EST-SSRs 的序列在所有三个时间点都是差异表达和共有的。此外，我们在几个抗性和易感草莓品种和野生物种中鉴定了一些 DE-EST-SSR 的等位基因多态性。我们研究中的EST-SSRs与草莓中报道的QTL抗性位点相关，该位点在C. fructicola感染后差异表达，并且在不同草莓品种和野生物种中具有等位基因多态性。这为在标记辅助育种中利用EST-SSRs以改善未来抗炭疽病育种提供了线索。我们研究中的EST-SSRs与草莓中报道的QTL抗性位点相关，该位点在C. fructicola感染后差异表达，并且在不同草莓品种和野生物种中具有等位基因多态性。这为在标记辅助育种中利用EST-SSRs以改善未来抗炭疽病育种提供了线索。我们研究中的EST-SSRs与草莓中报道的QTL抗性位点相关，该位点在C. fructicola感染后差异表达，并且在不同草莓品种和野生物种中具有等位基因多态性。这为在标记辅助育种中利用EST-SSRs以改善未来抗炭疽病育种提供了线索。