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Utilization of Morchella esculenta-mediated green synthesis golden nanoparticles in biomedicine applications
Preparative Biochemistry & Biotechnology ( IF 2.0 ) Pub Date : 2020-07-31 , DOI: 10.1080/10826068.2020.1799390
Hilal Acay 1
Affiliation  

Abstract

This study aimed to synthesize gold nanoparticles (AuNPs) by hot water extract in room conditions using edible Morchella esculenta (ME) and investigate the bioactive properties of the synthesized Morchella esculenta-based gold nanoparticles (ME-AuNPs). The characterization of the biologically synthesized ME-AuNPs was made using the ultraviolet–visible spectrophotometry, X-ray crystallography, scanning electron microscopy, Fourier transforms infrared spectroscopy, and energy dispersive X-ray spectrum methods. The ME-AuNPs, with a particle size of 16.51 nm, were found to have strong bioactive properties. The antioxidant activity of the ME-AuNPs attempted by metal chelating activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and the β-carotene linoleate model system. The activities at 10 mg/mL were 82, 85, and 77% for the chelation of ferrous ions, DPPH scavenging, and β-carotene linoleate tests, respectively. The ME-AuNPs also showed strong antimicrobial activity against various pathogen microorganisms and strong cytotoxic activity in the A549 and HepG2 cell lines. This study demonstrated the possibility of using a cheap and nontoxic fungal extract as a reducing and stabilizing agent for the synthesis of size-controlled, large-scale, and biocompatible AuNPs that could be used in future diagnostic and therapeutic applications.



中文翻译:

羊肚菌介导的绿色合成金纳米颗粒在生物医学应用中的利用

抽象的

这项研究旨在利用食用食用羊肚菌(ME)在室温下通过热水提取物合成金纳米颗粒(AuNPs),并研究合成的羊肚菌的生物活性。基金纳米颗粒(ME-AuNPs)。使用紫外可见分光光度法,X射线晶体学,扫描电子显微镜,傅立叶变换红外光谱和能量色散X射线光谱法对生物合成的ME-AuNPs进行了表征。发现ME-AuNPs的粒径为16.51 nm,具有很强的生物活性。通过金属螯合活性,2,2-二苯基-1-吡啶并肼基(DPPH)自由基清除能力和β-胡萝卜素亚油酸酯模型系统尝试了ME-AuNPs的抗氧化活性。10 mg / mL的亚铁离子螯合,DPPH清除和β-胡萝卜素亚油酸酯测试的活性分别为82%,85%和77%。在A549和HepG2细胞系中,ME-AuNPs还显示出对各种病原微生物的强大抗菌活性和强大的细胞毒性活性。这项研究表明,有可能使用廉价且无毒的真菌提取物作为还原剂和稳定剂,以合成尺寸可控制的,大规模的,生物相容的AuNPs,这些AuNPs可用于未来的诊断和治疗应用。

更新日期:2020-07-31
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