Leaf shape is an important agronomic trait for constructing an ideal plant type in rice, and high-density genetic map is facilitative in improving accuracy and efficiency for quantitative trait loci (QTL) analysis of leaf trait. In this study, a high-density genetic map contained 10,760 specific length amplified fragment sequencing (SLAF) markers was established based on 149 recombinant inbred lines (RILs) derived from the cross between Rekuangeng (RKG) and Taizhong1 (TN1), which exhibited 1,613.59 cM map distance with an average interval of 0.17 cM. A total of 24 QTLs were detected and explained the phenotypic variance ranged from 9% to 33.8% related to the leaf morphology across two areas. Among them, one uncloned major QTL qTLLW1 (qTLL1 and qTLLW1) involved in regulating leaf length and leaf width with max 33.8% and 22.5% phenotypic variance respectively was located on chromosome 1, and another major locus qTLW4 affecting leaf width accounted for max 25.3% phenotypic variance was mapped on chromosome 4. Fine mapping and qRT-PCR expression analysis indicated that qTLW4 may be allelic to NAL1 (Narrow leaf 1) gene.

叶形是构建水稻理想植物类型的重要农艺性状，高密度遗传图谱有助于提高叶性状的数量性状基因座（QTL）分析的准确性和效率。在这项研究中，基于来自Rekuangeng（RKG）和Taizhong1（TN1）之间杂交的149个重组自交系（RIL），建立了包含10,760个特定长度扩增片段测序（SLAF）标记的高密度遗传图谱，显示1,613.59 cM地图距离，平均间隔为0.17 cM。总共检测到24个QTL，并解释了与两个区域的叶片形态有关的表型变异范围从9％到33.8％。其中，一个未克隆的主要QTLqTLLW1 （qTLL1 和 qTLLW1）参与调节叶的长度和叶宽度，最大表型变异分别为33.8％和22.5％位于1号染色体上，是另一个主要基因座 qTLW4 影响叶宽的最大表型变异占25.3％，位于4号染色体上。精细定位和qRT-PCR表达分析表明： qTLW4 可能是等位基因 NAL1 （窄叶1）基因。