MicroRNA-126 (miR-126) is an endothelial-specific microRNA that has shown beneficial effects on endothelial dysfunction. However, the underlying molecular mechanism is unclear. The present study evaluated the effects of miR-126 on the cell migration and underlying mechanism in HUVECs treated with palmitate. The present results demonstrated that overexpression of miR-126 was found to decrease cell migration in palmitate-treated HUVECs, with decreased MLCK expression and subsequent decreased phosphorylated MLC level. miR-126 also decreased the phosphorylation of MYPT1 in palmitate-treated HUVECs. In addition, it was demonstrated that miR-126 decreases expression of the NADPH oxidase subunits, p67 and Rac family small GTPase 1 with a subsequent decrease in cell apoptosis. Moreover, the phosphorylation of ERK was reduced by miR-126 in palmitate-induced HUVECs. Taken together, the present study showed that the effect of miR-126 on cell migration and cell apoptosis is mediated through downregulation of MLCK via the ERK/MAPK pathway.

中文翻译：

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MicroRNA-126 通过 ERK/MAPK 通路下调肌球蛋白轻链激酶，调节 HUVEC 中棕榈酸诱导的迁移

MicroRNA-126 (miR-126) 是一种内皮特异性 microRNA，已显示出对内皮功能障碍的有益作用。然而，潜在的分子机制尚不清楚。本研究评估了 miR-126 对棕榈酸酯处理的 HUVEC 中细胞迁移和潜在机制的影响。目前的结果表明，发现 miR-126 的过表达减少了棕榈酸酯处理的 HUVEC 中的细胞迁移，MLCK 表达降低，随后磷酸化 MLC 水平降低。miR-126 还降低了棕榈酸酯处理的 HUVEC 中 MYPT1 的磷酸化。此外，已证明 miR-126 降低 NADPH 氧化酶亚基 p67 和 Rac 家族小 GTPase 1 的表达，随后细胞凋亡减少。而且，在棕榈酸酯诱导的 HUVEC 中，miR-126 降低了 ERK 的磷酸化。总之，本研究表明 miR-126 对细胞迁移和细胞凋亡的影响是通过 ERK/MAPK 通路下调 MLCK 介导的。