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Regulation of Trpm activation and calcium wave initiation during Drosophila egg activation.
Molecular Reproduction and Development ( IF 2.7 ) Pub Date : 2020-07-31 , DOI: 10.1002/mrd.23403
Qinan Hu 1 , Mariana F Wolfner 1
Affiliation  

The transition from a developmentally arrested mature oocyte to a developing embryo requires a series of highly conserved events, collectively known as egg activation. All of these events are preceded by a ubiquitous rise of intracellular calcium, which results from influx of external calcium and/or calcium release from internal storage. In Drosophila, this calcium rise initiates from the pole(s) of the oocyte by influx of external calcium in response to mechanical triggers. It is thought to trigger calcium responsive kinases and/or phosphatases, which in turn alter the oocyte phospho‐proteome to initiate downstream events. Recent studies revealed that external calcium enters the activating Drosophila oocyte through Trpm channels, a feature conserved in mouse. The local entry of calcium raises the question of whether Trpm channels are found locally at the poles of the oocyte or are localized around the oocyte periphery, but activated only at the poles. Here, we show that Trpm is distributed all around the oocyte. This requires that it thus be specially regulated at the poles to allow calcium wave initiation. We show that neither egg shape nor local pressure is sufficient to explain this local activation of Trpm channels.

中文翻译:

果蝇卵激活过程中 Trpm 激活和钙波启动的调节。

从发育停滞的成熟卵母细胞到发育中的胚胎的转变需要一系列高度保守的事件,统称为卵子激活。所有这些事件之前都是细胞内钙的普遍升高,这是由于外部钙的流入和/或内部储存的钙释放所致。在果蝇中,这种钙从卵母细胞的两极开始上升,这是由于外部钙的流入响应机械触发。人们认为它会触发钙反应激酶和/或磷酸酶,进而改变卵母细胞磷酸蛋白质组以启动下游事件。最近的研究表明,外部钙进入激活果蝇卵母细胞通过 Trpm 通道,这是小鼠保守的特征。钙的局部进入引发了一个问题,即 Trpm 通道是在卵母细胞的两极局部发现还是位于卵母细胞外围,但仅在两极被激活。在这里,我们表明 Trpm 分布在卵母细胞周围。这需要在两极对其进行特殊调节以允许钙波启动。我们表明,鸡蛋形状和局部压力都不足以解释 Trpm 通道的这种局部激活。
更新日期:2020-08-28
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