Methicillin-resistant Staphylococcus aureus (MRSA) can form biofilms, which prevents the penetration of antibiotics, decreasing their efficacy. This study investigated whether baicalein has synergistic antibacterial effects with linezolid in vivo. We cultivated MRSA 17546 biofilms on silicone implants and inserted them into the air pouches of rat models. The rats were treated with linezolid, baicalein, or a combination therapy for three consecutive days. All treatments reduced the number of colony-forming units (CFU) in the biofilms compared to the control (p < 0.05). However, by day two, the CFU counts were significantly lower in the combination group than in the individual treatment groups (p < 0.05). Histological analysis of the air pouches showed that the severity of the inflammatory cell infiltration was severe in the combination therapy group. In the combination group, the biofilm structure on the implant's surface was sparse and more free colonies could be seen by scanning electron microscopy (SEM); by day three, no obvious biofilm was observed. The serum levels of Staphylococcus enterotoxin A (SEA), C-reactive protein (CRP), and procalcitonin (PCT) were the lowest in the group where rats were treated with the combination of baicalein and linezolid (p < 0.05) compared to other groups. The results suggest that baicalein may inhibit the accessory gene regulator system, reducing the expression of SEA, thus lowering CRP and PCT levels. Furthermore, the inhibitory effect was more pronounced when baicalein was combined with linezolid. These results provide an important basis for the development of a new combination regimen to treat patients with biofilm-associated MRSA infections.

耐甲氧西林的金黄色葡萄球菌（MRSA）可以形成生物膜，从而阻止抗生素的渗透，从而降低其功效。这项研究调查了黄ba素在体内是否与利奈唑胺有协同的抗菌作用。我们在有机硅植入物上培养了MRSA 17546生物膜，并将其插入大鼠模型的气囊中。大鼠连续三天接受利奈唑胺，黄ical素或联合疗法治疗。与对照相比，所有处理均减少了生物膜中菌落形成单位（CFU）的数量（p  <0.05）。然而，由两个天，CFU计数显著在组合组中降低比单独治疗组（p <0.05）。气囊的组织学分析表明，在联合治疗组中，炎症细胞浸润的严重程度很严重。在组合组中，植入物表面的生物膜结构稀疏，并且通过扫描电子显微镜（SEM）可以看到更多的游离菌落。到第三天，没有观察到明显的生物膜。的血清水平葡萄球菌肠毒素A（SEA），C-反应蛋白（CRP），降钙素原和（PCT）是其中大鼠用黄芩素和利奈唑胺的组合（治疗的组中的最低p <0.05）。结果表明黄ical素可能抑制辅助基因调节系统，降低SEA的表达，从而降低CRP和PCT水平。此外，当黄ical素与利奈唑胺合用时，抑制作用更为明显。这些结果为开发新的治疗生物膜相关MRSA感染患者的联合治疗方案提供了重要依据。