Herein, an aldehyde group mediated in situ reduction approach is presented for preparation of silver nanoparticles anchored oxidized regenerated cellulose (Ag/ORC) microfibers. During the preparation, cellulose acetate (CA) microfibers are produced by electrospining and treated with heat and alkaline to form regenerated cellulose (RC) microfibers. After oxidating the RC microfibers by NaIO₄, the generated aldehyde groups on the microfibers are employed to in situ reduce Ag ions, and the formed Ag nanoparticles (AgNPs) are directly deposited on the surface of oxidized regenerated cellulose (ORC) microfibers. The structure and morphology of the composite microfibers are characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and X-ray diffraction (XRD). It is found that AgNPs with a diameter of approximately of 17.5 nm are well distributed on the ORC microfibers. Furthermore, the antibacterial activity of the prepared Ag/ORC microfibers towards Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) is assessed via disk diffusion method, live/dead fluorescent imaging assay, and SEM examination. The results show that the Ag/ORC microfibers exhibit excellent antibacterial activity, providing a promising antibacterial material.

在本文中，提出了醛基介导的原位还原方法，用于制备锚定的氧化还原再生纤维素（Ag / ORC）银纳米纤维。在制备过程中，通过静电纺丝生产醋酸纤维素（CA）超细纤维，并通过加热和碱处理形成再生的纤维素（RC）超细纤维。用NaIO ₄氧化RC超细纤维后，在超细纤维上产生的醛基被原位使用。还原Ag离子，然后将形成的Ag纳米颗粒（AgNPs）直接沉积在氧化再生纤维素（ORC）超细纤维的表面上。通过透射电子显微镜（TEM），扫描电子显微镜（SEM），能量色散谱（EDS）和X射线衍射（XRD）表征复合微纤维的结构和形态。发现直径约17.5nm的AgNPs在ORC微纤维上分布良好。此外，通过以下方法评估了制备的Ag / ORC超细纤维对大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抗菌活性。圆盘扩散法，活/死荧光成像分析和SEM检查。结果表明，Ag / ORC微纤维表现出优异的抗菌活性，提供了有希望的抗菌材料。